14-3-3 epsilon Antibody

Western Blot: 14-3-3 epsilon Antibody [NBP1-32695] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 epsilon antibody [N1C3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody

Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody [NBP1-32695] - Antibody was tested at 1:200 in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).

Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody [NBP1-32695] - Paraffin-embedded A549 xenograft, using 14-3-3 epsilon antibody at 1:500 dilution.

Western Blot: 14-3-3 epsilon Antibody [NBP1-32695] - Sample (20 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:10000.

Immunoprecipitation: 14-3-3 epsilon Antibody [NBP1-32695] - IP samples: HeLa whole cell extract A. 40 ug HeLa whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of YWHAE protein by 4 ug 14-3-3 epsilon antibody [N1C3] (NBP1-32695) 12 % SDS-PAGE The immunoprecipitated YWHAE protein was detected by 14-3-3 epsilon antibody [N1C3] (NBP1-32695) diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]

• Reactivity: Hu, Mu, Rt, Bv, Ch, Pm, Xp
• Applications: WB, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

14-3-3 epsilon Antibody Summary

• Immunogen: Full length human 14-3-3 epsilon Recombinant protein.
• Localization: Cytoplasm, Melanosome
• Predicted Species: Rat (98%), Chicken (100%), Xenopus (96%), Bovine (100%), Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: YWHAE
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (96%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for 14-3-3 epsilon Antibody

• 1433 epsilon
• 14-3-3 epsilon
• 14-3-3 protein epsilon
• FLJ45465
• FLJ53559,14-3-3E
• KCIP-1
• MDCR
• MDS
• mitochondrial import stimulation factor L subunit
• protein kinase C inhibitor protein-1
• tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilonpolypeptide
• YWHAE

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for 14-3-3 epsilon Antibody (NBP1-32695)(2)

Publications using NBP1-32695

• O'Sullivan D, Dowling P, Joyce H et al. A novel inhibitory anti-invasive MAb isolated using phenotypic screening highlights AnxA6 as a functionally relevant target protein in pancreatic cancer. Br. J. Cancer. Oct 24 2017 [PMID: 28881357] (IP, Human)
• Dowling P, Moran B, Mcauley E et al. Quantitative label-free mass spectrometry analysis of formalin-fixed, paraffin-embedded tissue representing the invasive cutaneous malignant melanoma proteome. Oncology Letters [PMID: 27899996] (IHC)

Bioinformatics

• Gene Symbol: YWHAE