JAM-B/VE-JAM Antibody

Images

 
Western Blot: JAM-B/VE-JAM Antibody [NBP1-32655] - A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell ...read more
Immunohistochemistry-Paraffin: JAM-B/VE-JAM Antibody [NBP1-32655] - Paraffin-embedded mouse small intestine, using antibody at 1:200 dilution. Image courtesy of Koji Taniguchi, Ph. D (Laboratory of Dr. Michael Karin, ...read more
Western Blot: JAM-B/VE-JAM Antibody [NBP1-32655] - Sample (30 ug of whole cell lysate) A: A431 12 % SDS PAGE, antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: JAM-B/VE-JAM Antibody [NBP1-32655] - Paraffin-embedded TOV-112D xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: JAM-B/VE-JAM Antibody [NBP1-32655] - Paraffin-embedded human colon, using antibody at 1:200 dilution.

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

JAM-B/VE-JAM Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human JAM-B. The exact sequence is proprietary.
Localization
Cell junction, tight junction, Cell membrane, Single-pass type I membrane protein
Predicted Species
Bovine (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
JAM2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (81%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for JAM-B/VE-JAM Antibody

  • C21orf43
  • CD322 antigen
  • CD322
  • JAM2
  • JAMB
  • JAM-B
  • JAM-BJAM-IT/VE-JAM
  • junctional adhesion molecule 2JAM-2
  • junctional adhesion molecule B
  • PRO245
  • Vascular endothelial junction-associated molecule
  • VE-JAM
  • VE-JAMVEJAMCD322

Background

Junctional adhesion molecule 2 (JAM2), also know as JAMB, is a member of the newly reported glycoprotein family of adhesion molecules found at intercellular junctions of endothelial cells and lymphocytes (human). In adult murine tissue JAM2 expression is restricted to high endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells in kidney. Clone CRAM-18 F26 has been reported to inhibit transendothelial migration (Johnson-Leger, C.A. et al., 2002).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol JAM2
Entrez