JAM-B/VE-JAM Antibody - BSA Free

Western Blot: JAM-B/VE-JAM Antibody [NBP1-32655] - Human tissue extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with JAM-B antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: JAM-B/VE-JAM Antibody [NBP1-32655] - Paraffin-embedded TOV-112D xenograft, using antibody at 1:100 dilution.

Immunohistochemistry-Paraffin: JAM-B/VE-JAM Antibody [NBP1-32655] - Paraffin-embedded human colon, using antibody at 1:200 dilution.

• Reactivity: Hu, Bv
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

JAM-B/VE-JAM Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human JAM-B/VE-JAM. The exact sequence is proprietary.
• Localization: Cell junction, tight junction, Cell membrane, Single-pass type I membrane protein
• Predicted Species: Bovine (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: JAM2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for JAM-B/VE-JAM Antibody - BSA Free

• C21orf43
• CD322 antigen
• CD322
• JAM2
• JAMB
• JAM-B
• JAM-BJAM-IT/VE-JAM
• junctional adhesion molecule 2JAM-2
• junctional adhesion molecule B
• PRO245
• Vascular endothelial junction-associated molecule
• VE-JAM
• VE-JAMVEJAMCD322

Background

Junctional adhesion molecule 2 (JAM2), also know as JAMB, is a member of the newly reported glycoprotein family of adhesion molecules found at intercellular junctions of endothelial cells and lymphocytes (human). In adult murine tissue JAM2 expression is restricted to high endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells in kidney. Clone CRAM-18 F26 has been reported to inhibit transendothelial migration (Johnson-Leger, C.A. et al., 2002).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for JAM-B/VE-JAM Antibody (NBP1-32655)(1)

Publication using NBP1-32655

• Hintermann E, Bayer M, Conti CB et al. Junctional adhesion molecules JAM-B and JAM-C promote autoimmune-mediated liver fibrosis in mice J. Autoimmun. 2018-05-09 [PMID: 29753567] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: JAM2