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HAF017). Specific bands were detected for JAM-B/VE-JAM at approximately 43-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
HAF017). Specific bands were detected for JAM-B/VE-JAM at approximately 40-48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox" target="_blank">
1074-VJ), immobilized onto a microplate previously coated with Goat Anti-Human IgG Fc (Catalog # G-102-C), supports the adhesion of the J45.01 human acute lymphoblastic leukemia T lymphocyte cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Human JAM-B/VE-JAM Fc Chimera (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse JAM-B/VE-JAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1074). The ND50 is typically 0.2-0.8 µg/mL." class="big_lightbox" target="_blank">
988-VJ), immobilized onto a microplate previously coated with Goat Anti-Human IgG Fc (Catalog # G-102-C), supports the adhesion of the J45.01 human acute lymphoblastic leukemia T lymphocyte cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Mouse JAM‑B/VE‑JAM Fc Chimera (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse JAM-B/VE-JAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF988). The ND50 is typically 0.6-3.0 µg/mL." class="big_lightbox" target="_blank">
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types and may play a role in lymphocyte homing to secondary lymphoid organs.
![Western Blot JAM-B/VE-JAM Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/JAMB_AF1074_Western_Blot_21290.jpg)
![Western Blot JAM-B/VE-JAM Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/JAMB_AF1074_Western_Blot_21369.jpg)
![Immunohistochemistry JAM-B/VE-JAM Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/JAMB_AF1074_Immunohistochemistry_21912.jpg)
![Simple Western JAM-B/VE-JAM Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/JAMB_AF1074_Simple_Western_21685.jpg)
![Neutralization JAM-B/VE-JAM Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/JAM-B_AF1074_Block_Neutralize_8754.jpg)
