JAM-A Antibody

Western Blot: JAM-A Antibody [NBP1-49908] - Samples: Whole cell lysate from 293T (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-JAM-A antibody NBP1-49908 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. JAM-A was not successfully immunoprecipitated by rabbit anti-JAM-A antibody NBP1-49907, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).

Immunohistochemistry-Paraffin: JAM-A Antibody [NBP1-49908] - Human prostate carcinoma. Antibody: Affinity purified rabbit anti-JAM-A used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

JAM-A Antibody Summary

• Description: Novus Biologicals Rabbit JAM-A Antibody (NBP1-49908) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-JAM-A Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 249 and 299 of human Junctional Adhesion Molecule A using the numbering given in entry NP_058642.1 (GeneID 50848).
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: F11R
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34293456)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for JAM-A Antibody

• CD321 antigen
• CD321
• F11 receptor
• F11R
• JAM-1
• JAM1CD321
• JAMA
• JAM-A
• JCAMJAM
• Junctional adhesion molecule 1Platelet F11 receptor
• junctional adhesion molecule A
• PAM-1
• PAM-1KAT
• Platelet adhesion molecule 1

Background

Junctional Adhesion Molecules (JAMs) are components and regulators of the well-characterized epithelial and endothelial tight junction. JAM is selectively concentrated at intercellular junctions of endothelial and epithelial cells of different origins. Microscopy shows that JAM codistributes with tight junction components at the apical region of the intercellular cleft. A mAb directed to JAM (BV11) was found to inhibit spontaneous and chemokine-induced monocyte transmigration through an endothelial cell monolayer in vitro. Systemic treatment of mice with BV11 mAb blocked monocyte infiltration upon chemokine administration in subcutaneous air pouches (1). The human protein shares a highly conserved structure and sequence with the murine protein. However it is distinct in that it is constitutively expressed on circulating neutrophils, monocytes, platelets and lymphocyte subsets (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for JAM-A Antibody (NBP1-49908)(1)

Publication using NBP1-49908

• Pan Z, Gao Y, Liu S et al. Wu-Zi-Yan-Zong-Wan protects mouse blood-testis barrier from Tripterygium wilfordii Hook. f. multiglycoside-induced disruption by regulating proinflammatory cytokines Journal of ethnopharmacology 2021-07-19 [PMID: 34293456] (WB)

Bioinformatics

• Gene Symbol: F11R