Jak2 Antibody (OTI7D12) - Azide and BSA Free

Immunohistochemistry: Jak2 Antibody (OTI7D12) - Azide and BSA Free [NBP2-71056] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: Jak2 Antibody (OTI7D12) - Azide and BSA Free [NBP2-71056] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min)

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clone: OTI7D12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Jak2 Antibody (OTI7D12) - Azide and BSA Free Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 297-596 of human JAK2(NP_004963) produced in E.coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: JAK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Jak2 Antibody (OTI7D12) - Azide and BSA Free

• EC 2.7.10
• EC 2.7.10.2
• Jak2
• JAK-2
• Janus kinase 2Janus kinase 2 (a protein tyrosine kinase)
• JTK10
• tyrosine-protein kinase JAK2

Background

Jak2 is a member of the Janus family tyrosine kinases (Jaks), whose function is to transduce extracellular signals from cytokines and interferons. Jaks associate with cytokine receptors; upon ligand binding, Jaks phosphorylate the receptor. The phosphorylated receptors are bound by SH2-containing proteins, one class of which is the signal transducers and activators of transcription (STATs). Activated STATs, then, translocate to the nucleus to mediate gene transcription (1,2). Tyr1007/1008 of Jak2 are the homologous Tyr1054/1055 in Tyk2, and phosphorylation at these residues is needed for Tyk2 activation (3). A chromosomal aberration involving Jak2 is found in a form of pre-B acute myeloid leukemia (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: JAK2