ITPK1 Antibody (2C8) - Azide and BSA Free

Western Blot: ITPK1 Antibody (2C8) [H00003705-M01] - Analysis of ITPK1 expression in transfected 293T cell line by ITPK1 monoclonal antibody (M01), clone 2C8.Lane 1: ITPK1 transfected lysate(35.6 KDa).Lane 2: Non-transfected lysate.

Western Blot: ITPK1 Antibody (2C8) [H00003705-M01] - Analysis of ITPK1 over-expressed 293 cell line, cotransfected with ITPK1 Validated Chimera RNAi ( Cat # H00003705-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ITPK1 monoclonal antibody (M01), clone 2C8 (Cat # H00003705-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Sandwich ELISA: ITPK1 Antibody (2C8) [H00003705-M01] - Detection limit for recombinant GST tagged ITPK1 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, KD
• Clone: 2C8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ITPK1 Antibody (2C8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ITPK1 (AAH03622, 1 a.a. ~ 314 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MQTFLKGKRVGYWLSEKKIKKLNFQAFAELCRKRGMEVVQLNLSRPIEEQGPLDVIIHKLTDVILEADQNDSQSLELVHRFQEYIDAHPETIVLDPLPAIRTLLDRSKSYELIRKIEAYMEDDRICSPPFMELTSLCGDDTMRLLEKNGLTFPFICKTRVAHGTNSHEMAIVFNQEGLNAIQPPCVVQNFINHNAVLYKVFVVGESYTVVQRPSLKNFSAGTSDRESIFFNSHNVSKPESSSVLTELDKIEGVFERPSDEVIRELSRALRQALGVSLFGIDIIINNQTGQHAVIDINAFPGDCQVCFIEGWKTD
• Specificity: ITPK1 (2C8)
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITPK1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500RNAi Validation
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ITPK1 Antibody (2C8) - Azide and BSA Free

• EC 2.7.1.134
• EC 2.7.1.159
• inositol 13,4-triphosphate 5/6 kinase
• inositol 13,4-trisphosphate 5/6 kinase
• Inositol 13,4-trisphosphate 5/6-kinase
• inositol-tetrakisphosphate 1-kinase
• Inositol-triphosphate 5/6-kinase
• Ins(13,4)P(3) 5/6-kinase
• ITRPK1

Background

Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca(2+)-activated Cl(-) channels, while Ins(1,3,4,5,6)P5 is not. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway. Also acts as an inositol polyphosphate phosphatase that dephosphorylate Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3, and Ins(1,3,4,5,6)P5 to Ins(3,4,5,6)P4. May also act as an isomerase that interconverts the inositol tetraphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium. Probably acts as the rate-limiting enzyme of the InsP6 pathway. Modifies TNF-alpha-induced apoptosis by interfering with the activation of TNFRSF1A-associated death domain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITPK1
• Entrez:
  • 3705(Human)
• OMIM:
  • 601838(Human)
• Uniprot:
  • AAH03622(Human)