Islet-1 Antibody (CL11651) - Azide and BSA Free

Staining of HeLa cells using the Anti-Islet-1 monoclonal antibody, showing localization to nucleoplasm and cytosol in green. Microtubule probe is visualized in red.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: CL11651
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Islet-1 Antibody (CL11651) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a synthetic peptide of P61371, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ISL1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
• Application Notes: Immunocytochemistry/Immunofluorescence, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Islet-1 Antibody (CL11651) - Azide and BSA Free

• insulin gene enhancer protein ISL-1
• ISL LIM homeobox 1
• ISL1 transcription factor, LIM/homeodomain
• ISL1 transcription factor, LIM/homeodomain, (islet-1)
• ISL1
• Isl-1
• Islet1
• Islet-1

Background

Islet 1 encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ISL1