Islet-1 Antibody (2E7)

Images

 
Western Blot: Islet-1 Antibody (2E7) [H00003670-M05] - Analysis of ISL1 expression in PC-12 (Cat # L012V1).
Immunocytochemistry/ Immunofluorescence: Islet-1 Antibody (2E7) [H00003670-M05] - Analysis of monoclonal antibody to ISL1 on HeLa cell. Antibody concentration 10 ug/ml

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Func, ICC/IF, IP, ChIP
Clone
2E7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Islet-1 Antibody (2E7) Summary

Immunogen
ISL1 (NP_002193, 63 a.a. ~ 159 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YCKRDYIRLYGIKCAKCSIGFSKNDFVMRARSKVYHIECFRCVACSRQLIPGDEFALREDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAEP
Localization
Nuclear, occasionally cytoplasmic
Specificity
ISL1 - ISL1 transcription factor, LIM/homeodomain, (islet-1)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ISL1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA
  • Functional
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
  • Western Blot 1:500
Application Notes
This antibody reactivity against cell lysate and recombinant protein for WB. It has been useful for IF and ELISA. IP usage reported in scientific literature (PMID: 25070240). Use in chromatin immunoprecipitation reported in scientific literature (PMID: 26635867).
Publications
Read Publications using H00003670-M05.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Islet-1 Antibody (2E7)

  • insulin gene enhancer protein ISL-1
  • ISL LIM homeobox 1
  • ISL1 transcription factor, LIM/homeodomain
  • ISL1 transcription factor, LIM/homeodomain, (islet-1)
  • ISL1
  • Isl-1
  • Islet1
  • Islet-1

Background

This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Islet-1 Antibody (H00003670-M05)(7)

We have publications tested in 3 applications: ChIP, IP, WB.


Filter By Application
ChIP
(1)
IP
(1)
WB
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ISL1
Entrez
OMIM
Uniprot