Islet-1 Antibody (1H9)

Western Blot: Islet-1 Antibody (1H9) [NBP1-47537] - Analysis using ISL1 mouse mAb against full-length ISL1 (aa1-349)-hIgGFc transfected HEK293 cell lysate(1).

Immunocytochemistry/Immunofluorescence: Islet-1 Antibody (1H9) [NBP1-47537] - Analysis of HEK293 cells trasfected with full-length ISL1-hIgGFc using ISL1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry-Paraffin: Islet-1 Antibody (1H9) [NBP1-47537] - Analysis of human lung cancer (left) and cervical carcinoma (right), showing nuclear localization using ISL1 mouse mAb with DAB staining.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 1H9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Islet-1 Antibody (1H9) Summary

• Immunogen: Purified recombinant fragment of human Islet-1 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ISL1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is useful in Western Blot, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunofluorescence, ELISA.
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Adrenal Whole Tissue Lysate (Adult Whole Diabetes)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Islet-1 Antibody (1H9)

• insulin gene enhancer protein ISL-1
• ISL LIM homeobox 1
• ISL1 transcription factor, LIM/homeodomain
• ISL1 transcription factor, LIM/homeodomain, (islet-1)
• ISL1
• Isl-1
• Islet1
• Islet-1

Background

ISL1 (ISL1 transcription factor, LIM/homeodomain) is a member of the LIM/homeodomain family of transcription factors. It binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. It is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Islet-1 expression defines cardiac progenitor cell populations and is required for normal cardiac development and asymmetry. Mutations in this gene have been associated with maturity-onset diabetes of the young.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ISL1
• Entrez:
  • 3670(Human)
• Uniprot:
  • P61371()