IRF6 Overexpression Lysate (Native)

Images

 
Western Blot: IRF6 Overexpression Lysate (Adult Normal) [NBL1-12036] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IRF6.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

IRF6 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201579 and based on accession number NM_006147. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens interferon regulatory factor 6 (IRF6), mRNA.
Gene
IRF6

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
52.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for IRF6

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for IRF6 Overexpression Lysate (Native)

  • interferon regulatory factor 6
  • IRF6
  • IRF-6
  • LPS
  • OFC6
  • OFC6PPS
  • PIT
  • PPS
  • Van der Woude syndrome
  • VWS
  • VWS1

Background

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. This protein is involved in palate formation. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-89306
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NB100-56566
Species: Hu, Mu, Rt
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, CyTOF-ready, Flow-CS, Flow-IC
NB100-74413
Species: Hu, Mu, Rb
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
PP-H4417-00
Species: Hu
Applications: WB, IP, DirELISA
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NBP2-33090
Species: Hu, Mu, Rt, Bv
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
NBP1-32108
Species: Hu, Rt
Applications: WB, ICC/IF
NBP2-45897
Species: Hu
Applications: WB, IHC
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
AF1195
Species: Hu
Applications: WB, IHC, Neut
NBP1-92172
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56605
Species: Av, Bv, Sh
Applications: WB
NBP1-26642
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP

Publications for IRF6 Lysate (NBL1-12036) (0)

There are no publications for IRF6 Lysate (NBL1-12036).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IRF6 Lysate (NBL1-12036) (0)

There are no reviews for IRF6 Lysate (NBL1-12036). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IRF6 Lysate (NBL1-12036) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IRF6 Products

Bioinformatics Tool for IRF6 Lysate (NBL1-12036)

Discover related pathways, diseases and genes to IRF6 Lysate (NBL1-12036). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IRF6 Lysate (NBL1-12036)

Discover more about diseases related to IRF6 Lysate (NBL1-12036).
 

Pathways for IRF6 Lysate (NBL1-12036)

View related products by pathway.

PTMs for IRF6 Lysate (NBL1-12036)

Learn more about PTMs related to IRF6 Lysate (NBL1-12036).

Blogs on IRF6

There are no specific blogs for IRF6, but you can read our latest blog posts.

Customers Who Bought This Also Bought

IRF6 Antibody
NBP1-51911

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IRF6 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol IRF6
Entrez