IRAK2 Antibody (JG36-23)

Western Blot: IRAK2 Antibody (JG36-23) [NBP2-76843] - Western blot analysis of IRAK2 on different cell lysates using anti-IRAK2 at 1/500 dilution.Positive control:Line 1: PC-3M Line 2: A549Line 3: HepG2

Immunocytochemistry/Immunofluorescence: IRAK2 Antibody (JG36-23) [NBP2-76843] - ICC staining IRAK2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: IRAK2 Antibody (JG36-23) [NBP2-76843] - Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.

Flow Cytometry: IRAK2 Antibody (JG36-23) [NBP2-76843] - Flow cytometric analysis of Jurkat cells with IRAK2 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry: IRAK2 Antibody (JG36-23) [NBP2-76843] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.

Immunohistochemistry: IRAK2 Antibody (JG36-23) [NBP2-76843] - Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.

Product Discontinued

IRAK2 Antibody (JG36-23) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human IRAK2 aa 30-270 / 625. (SwissProt: O43187 Human)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IRAK2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IRAK2 Antibody (JG36-23)

• interleukin-1 receptor associated kinase-2
• interleukin-1 receptor-associated kinase 2
• interleukin-1 receptor-associated kinase-like 2
• IRAK2
• IRAK-2
• MGC150550

Background

IRAK2 encodes the interleukin-1 receptor-associated kinase 2, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. IRAK2 is reported to participate in the IL1-induced upregulation of NF-kappaB. The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IRAK2