IQGAP1 Antibody - BSA Free

Western Blot: IQGAP1 Antibody [NBP1-83143] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry-Paraffin: IQGAP1 Antibody [NBP1-83143] - Staining in human endometrium and skeletal muscle tissues using anti-IQGAP1 antibody. Corresponding IQGAP1 RNA-seq data are presented for the same tissues.

Western Blot: IQGAP1 Antibody [NBP1-83143] - Analysis in human cell line A-431.

Immunohistochemistry-Paraffin: IQGAP1 Antibody [NBP1-83143] - Staining of human endometrium shows high expression.

Immunohistochemistry-Paraffin: IQGAP1 Antibody [NBP1-83143] - Staining of human skeletal muscle shows low expression as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

IQGAP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit IQGAP1 Antibody - BSA Free (NBP1-83143) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: NRALESGDVNTVWKQLSSSVTGLTNIEEENCQRYLDELMKLKAQAHAENNEFITWNDIQACVDHVNLVVQEEHERILAIGLINEALDEGDAQKTLQALQIPAAKLEGVLAEVAQHYQDTLIRAKREKAQEIQDESAVLWLDEIQGG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IQGAP1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  IQGAP1 Protein (NBP1-83143PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for IQGAP1 Antibody - BSA Free

• HUMORFA01
• IQ motif containing GTPase activating protein 1
• IQGAP1
• KIAA0051ras GTPase-activating-like protein IQGAP1
• p195
• p195RasGAP-like with IQ motifs
• SAR1

Background

IQGAP1 encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IQGAP1