IQGAP1 Antibody

Western Blot: IQGAP1 Antibody [NBP1-06529] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IQGAP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry-Paraffin: IQGAP1 Antibody [NBP1-06529] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-IQGAP1 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Immunoprecipitation: IQGAP1 Antibody [NBP1-06529] - Detection of human IQGAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IQGAP1 antibody NBP1-06529 (lot NBP1-06529-2) used for IP at 3 ug per reaction. IQGAP1 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-06529-1). For blotting immunoprecipitated IQGAP1, NBP1-06529 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

IQGAP1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human motif containing GTPase activating protein 1 using the numbering given in entry NP_003861.1 (GeneID 8826).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IQGAP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IQGAP1 Antibody

• HUMORFA01
• IQ motif containing GTPase activating protein 1
• IQGAP1
• KIAA0051ras GTPase-activating-like protein IQGAP1
• p195
• p195RasGAP-like with IQ motifs
• SAR1

Background

This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IQGAP1 Antibody (NBP1-06529)(3)

Publications using NBP1-06529

• Kumar A, Larrea D, Pero ME et al. MFN2-dependent recruitment of ATAT1 coordinates mitochondria motility with alpha-tubulin acetylation and is disrupted in CMT2A bioRxiv : the preprint server for biology 2023-03-16 [PMID: 36993604] (KO, ICC/IF, WB, Mouse)
• Mosaddeghzadeh N, Pudewell S, Bazgir F et al. CDC42-IQGAP Interactions Scrutinized: New Insights into the Binding Properties of the GAP-Related Domain International journal of molecular sciences 2022-08-09 [PMID: 36012107] (WB, Human)
• Sun H, Kapuria V, Peterson LF et al. Bcr-Abl ubiquitination and Usp9x inhibition block kinase signaling and promote CML cell apoptosis. Blood 2011-03-01 [PMID: 21248063]

Review for IQGAP1 Antibody (NBP1-06529) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 09/15/2015

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: IQGAP1
• Entrez:
  • 8826(Human)
• Uniprot:
  • NP_003861.1(Human)