IQGAP1 Antibody (JE50-66)

Western Blot: IQGAP1 Antibody (JE50-66) [NBP3-32479] - Western blot analysis of IQGAP1 on different lysates with Rabbit anti-IQGAP1 antibody (NBP3-32479) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: RAW264.7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 189 kDa Observed band size: 189 kDa Exposure time: 1 minute 40 second; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32479) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: IQGAP1 Antibody (JE50-66) [NBP3-32479] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-IQGAP1 antibody (NBP3-32479) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32479) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: IQGAP1 Antibody (JE50-66) [NBP3-32479] - Immunocytochemistry analysis of HeLa cells labeling IQGAP1 with Rabbit anti-IQGAP1 antibody (NBP3-32479) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IQGAP1 antibody (NBP3-32479) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Flow Cytometry: IQGAP1 Antibody (JE50-66) [NBP3-32479] - Flow cytometric analysis of HeLa cells labeling IQGAP1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32479, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: JE50-66
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

IQGAP1 Antibody (JE50-66) Summary

• Description: Novus Biologicals Rabbit IQGAP1 Antibody (JE50-66) (NBP3-32479) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human IQGAP1 aa 1-50 / 1,657. (Uniprot: P46940)
• Localization: Cell membrane, Nucleus, Cytoplasm, Apical cell membrane, Basolateral cell membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IQGAP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:4000
  • Western Blot 1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IQGAP1 Antibody (JE50-66)

• HUMORFA01
• IQ motif containing GTPase activating protein 1
• IQGAP1
• KIAA0051ras GTPase-activating-like protein IQGAP1
• p195
• p195RasGAP-like with IQ motifs
• SAR1

Background

IQGAP1 encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IQGAP1