Involucrin Antibody - BSA Free

Western Blot: Involucrin Antibody [NBP2-16981] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Involucrin antibody [N1N2], N-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Involucrin Antibody [NBP2-16981] - Immunohistochemical analysis of paraffin-embedded H661 xenograft, using antibody at 1:250 dilution.

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Involucrin antibody [N1N2], N-term (NBP2-16981) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Involucrin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Involucrin Antibody - BSA Free (NBP2-16981) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Involucrin Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Involucrin. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IVL
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Involucrin Antibody - BSA Free

• involucrin

Background

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Involucrin Antibody (NBP2-16981)(2)

Publications using NBP2-16981

• Qiao W, Xie T, Lu J et Al. Identification of potential hub genes associated with atopic dermatitis-like recombinant human epidermal model using integrated transcriptomic and proteomic analysis Biomol Biomed 2024-01-12 [PMID: 37540585]
• Berard AR, Brubaker DK, Birse K et al. Vaginal epithelial dysfunction is mediated by the microbiome, metabolome, and mTOR signaling Cell reports 2023-05-05 [PMID: 37149863] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: IVL
• Uniprot:
  • P07476(Human)