Involucrin Antibody (SY8) - BSA Free

• Reactivity: Hu, Po, Ca, Pm
• Applications: WB, ICC/IF, IHC, IP
• Clone: SY8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Involucrin Antibody (SY8) - BSA Free Summary

• Immunogen: Purified involucrin from human keratinocytes.
• Localization: Cytoplasmic
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IVL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Involucrin Antibody (SY8) - BSA Free

• involucrin

Background

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions and is reduced in severe dysplasias of the larynx and cervix. Its gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Involucrin Antibody (NB100-2774)(3)

Publications using NB100-2774

• Hazawa M, Ikliptikawati D, Iwashima Y et al. Super-enhancer trapping by the nuclear pore via intrinsically disordered regions of proteins in squamous cell carcinoma cells Cell Chemical Biology 2023-11-01 [PMID: 37924814] (IHC-P, Human)
• Peramo A, Marcelo CL. Visible effects of rapamycin (sirolimus) on human skin explants in vitro Arch Dermatol Res 2012-09-08 [PMID: 22960739] (IF/IHC, Human)
• Peramo A, Feinberg SE, Marcelo CL. A putative in vitro organotypic model of molting with human skin explants. Arch Dermatol Res;304(2):145-53. 2012-03-01 [PMID: 22037627] (IF/IHC, Human)

Review for Involucrin Antibody (NB100-2774) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: David Krull IHC-P Human 12/08/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: human skin
• Species: Human

Bioinformatics

• Gene Symbol: IVL
• Entrez:
  • 3713(Human)