INTS6 Antibody

Western Blot: INTS6 Antibody [NB100-86990] - Detection of Human INT6 NB100-86990. INT6 was also immunoprecipitated by another rabbit anti-INT6 antibody.

Immunohistochemistry-Paraffin: INTS6 Antibody [NB100-86990] - Human thyroid carcinoma. Antibody used at a dilution of 1:200 (1ug/ml).

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

INTS6 Antibody Summary

• Description: Novus Biologicals Rabbit INTS6 Antibody (NB100-86990) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-INTS6 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 750 and 800 of human integrator complex subunit 6 using the numbering given in entry NP_036273.1 (GeneID 26512).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: INTS6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for INTS6 Antibody

• DBI1
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 26
• DICE1DBI-1DDX26ADDX26DEAD box protein
• DKFZp434B105
• HDB
• INT6
• integrator complex subunit 6
• Notchl2
• Protein DDX26
• Protein deleted in cancer 1
• RNA helicase HDB

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. The protein encoded by this gene is a DEAD box protein that is part of a complex that interacts with the C-terminus of RNA polymerase II and is involved in 3' end processing of snRNAs. In addition, this gene is a candidate tumor suppressor and located in the critical region of loss of heterozygosity (LOH). Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for INTS6 Antibody (NB100-86990)(1)

Publication using NB100-86990

• Kirstein N, Dokaneheifard S, Cingaram PR et al. The Integrator complex regulates microRNA abundance through RISC loading Science advances 2023-02-10 [PMID: 36763664] (WB, Human)

Bioinformatics

• Gene Symbol: INTS6
• Entrez:
  • 26512(Human)
• Uniprot:
  • NP_036273.1(Human)