Integrin beta 5 Antibody [Alexa Fluor® 532]

• Reactivity: Mu, Rt
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 532

Integrin beta 5 Antibody [Alexa Fluor® 532] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin β5
  Gly24-Asn719
  Accession # NP_001139356
• Specificity: Detects mouse and rat in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 5 Antibody [Alexa Fluor® 532]

• FLJ26658
• Integrin beta 5
• integrin beta-5
• integrin, beta 5
• ITGB5

Background

The beta 5 integrin is a 95-110 kDa member of the integrin beta chain family, integrin superfamily of molecules. It is expressed by select cell types, including microglia, keratinocytes, fibroblasts, endothelial cells, adhesive monocytes and macrophages, retinal pigment epithelium, immature dendritic cells and colonic epithelium. The Integrin beta 5 subunit forms a noncovalent heterodimer with the alpha v integrin subunit, and together they bind vitronectin, and the Thy-1 antigen. alpha v beta 5 is reported to participate in phagocytosis, and act in concert with CD81, CD36 and stabilin-2 on the cell surface. When alpha v beta 5 binds Thy-1, the heterodimer apparently interfers with cell membrane activation of TGF-beta . Intracellularly, it appears to regulate actin skeleton remodeling via focal adhesion complex formation, and may contribute to cryptic pathways associated with antigen presentation. Mature mouse Integrin beta 5 is a 775 amino acid (aa) type I transmembrane glycoprotein that contains a 696 aa extracellular domain (ECD) (aa 24-719) and a 56 aa cytoplasmic region. The ECD contains a 243 aa vWF-A domain (aa 136-378) and a Cys-rich tandem repeat region (aa 465-630). There is one splice variant that shows a 57 aa substitution for aa 760-798. This splice form does not appear to be expressed by the human Integrin beta 5 gene. Over aa 24-719, mouse Integrin beta 5 shares 91% and 97% aa sequence identity with human and rat Integrin beta 5, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.