Integrin beta 2/CD18 Antibody [Alexa Fluor® 488]

• Reactivity: Mu, Ca
• Applications: WB, Flow, CyTOF-ready, Func
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

Integrin beta 2/CD18 Antibody [Alexa Fluor® 488] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin β2
  Gln24-Asn702
  Accession # P11835
• Specificity: Detects mouse Integrin beta 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant human Integrin beta 2 and recombinant mouse (rm) Integrin beta 6 is observed and less than 5%
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Adhesion Activation
  • CyTOF-ready
  • Flow Cytometry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 2/CD18 Antibody [Alexa Fluor® 488]

• 959 beta subunit precursor)10
• CD18 antigen
• CD18
• CD18beta 2
• cell surface adhesion glycoprotein (LFA-1
• CR3
• Integrin beta 2
• integrin beta-2
• integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit)
• integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
• ITGB2
• LAD
• LCAMB
• leukocyte cell adhesion molecule CD18
• leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C
• LFA-1 beta
• LFA-1
• MAC-1 beta
• MF17
• MFI7
• P150
• p150,95 beta

Background

Mouse Integrin beta 2 is a transmembrane glycoprotein with an 85 kDa molecular weight (predicted) and is expressed by all leukocytes. The Integrin beta chain can be found in heterodimeric complexes with Integrin subunits alpha D, alpha L (complex also known as LFA-1), alpha M (Mac-1), or alpha X (CR-4) leading to different Integrin proteins. The extra cellular domains of mouse and canine Integrin beta 2 share an 80.3% aa sequence identity. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling and leukocyte trafficking.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P11835()