Integrin alpha V/CD51 Antibody (273210) [Unconjugated]

Integrin aV/CD51 was detected in immersion fixed rat mesenchymal stem cells using Mouse Anti-Human Integrin aV/ CD51 Monoclonal Antibody (Catalog # MAB12191) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Huh‑7 human hepatoma cell line were stained with Mouse Anti-Human Integrin alpha V/CD51 Monoclonal Antibody (Catalog # MAB12191, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 273210
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin alpha V/CD51 Antibody (273210) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha V/CD51
  Phe31-Val992
  Accession # NP_002201
• Specificity: Detects human Integrin alpha V/CD51 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGAV
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha V/CD51 Antibody (273210) [Unconjugated]

• antigen identified by monoclonal L230
• CD51 antigen
• CD51
• Integrin alpha V
• integrin alpha-V
• integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
• ITGAV
• MSK8
• Vitronectin Receptor alpha
• Vitronectin receptor subunit alpha
• VNRA
• VNRADKFZp686A08142

Background

Integrin alpha V (CD51; also vitronectin receptor subunit alpha) is a 140‑150 kDa member of the integrin alpha -chain family of adhesion molecules. It forms disulfide-linked integral membrane heterodimers with at least five beta -chains, including beta 1, 3, 5, 6 and 8. Human  alpha V is a 1018 aa type I transmembrane glycoprotein that contains a 962 aa extracellular domain (ECD) and a short 32 aa cytoplasmic tail. The ECD contains seven FG (PheAlaGly)-GAP (GlyAlaPro) repeats that form a beta -propellor domain (aa 46‑483). Furin cleavage of the alpha V ECD occurs after Gly889, generating a disulfide-linked heteromeric subunit alpha V chain. alpha V-containing integrins bind multiple ECM molecules, including vitronectin, osteopontin, MMP-2 and TSP. The ECD of human alpha V shares 92% and 90% aa sequence identity with mouse and rat alpha V ECD, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha V/CD51 Antibody (MAB12191)(4)

Publications using MAB12191

• Bingjie Jiang, Yuewei Xu, Yihe Zhang, Meng Huee Lee Translocating a High-Affinity Designer TIMP-1 to the Cell Membrane for Total Renal Carcinoma Inhibition: Putting the Prion Protein to Good Use Molecular and Cellular Biology 2019-09-01 [PMID: 31208977]
• Allison Alwan TerBush, Florianne Hafkamp, Hee Jun Lee, Laurent Coscoy A Kaposi's Sarcoma-Associated Herpesvirus Infection Mechanism Is Independent of Integrins alpha 3 beta 1, alpha V beta 3, and alpha V beta 5 Journal of Virology 2018-09-01 [PMID: 29899108]
• Allison Alwan TerBush, Florianne Hafkamp, Hee Jun Lee, Laurent Coscoy A Kaposi's Sarcoma-Associated Herpesvirus Infection Mechanism Is Independent of Integrins alpha 3 beta 1, alpha V beta 3, and alpha V beta 5 Journal of Virology 2018-09-01 [PMID: 29899108] (Immunocytochemistry, Human)
• Stephanie R. Gillespie, Liana J. Tedesco, Lingyan Wang, Audrey M. Bernstein The deubiquitinase USP10 regulates integrin beta1 and beta5 and fibrotic wound healing Journal of Cell Science 2017-10-15 [PMID: 28851806]

Review for Integrin alpha V/CD51 Antibody (MAB12191) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IF Human 02/04/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 22274920
• Species: Human

Bioinformatics

• Gene Symbol: ITGAV
• Uniprot:
  • NP_002201()