Integrin alpha L/CD11a Antibody (M594)

Western Blot: Integrin alpha L/CD11a Antibody (M594) [NBP3-23395] - Western blot analysis of human Jurkat whole cell lysate. The blot was probed with mouse monoclonal anti-Integrin alpha L/CD11a (NBP3-23395) at a dilution of 1:500 (lane 1), 1:1000 (lane 2), 1:2000 (lane 3) and 1:4000 (lane 4).

Product Discontinued

Integrin alpha L/CD11a Antibody (M594) Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Clone M594 was generated from a recombinant protein containing amino acid residues in the extracellular region of human Integrin alpha L/CD11a. This sequence has low homology to other integrin family members. [UniProt# P20701]
• Specificity: The antibody detects a 180 kDa protein corresponding to the molecular mass of Integrin alpha L/CD11a on SDS-PAGE immunoblots of human Jurkat cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGAL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
• Theoretical MW: 180 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for Integrin alpha L/CD11a Antibody (M594)

• CD11 antigen-like family member A
• CD11a antigen
• CD11a
• CD11Aantigen CD11A (p180), lymphocyte function-associated antigen 1, alphapolypeptide
• Integrin alpha L
• integrin alpha-L
• integrin gene promoter
• integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen1; alpha polypeptide)
• ITGAL
• Leukocyte adhesion glycoprotein LFA-1 alpha chain
• Leukocyte function-associated molecule 1 alpha chain
• LFA-1 alpha
• LFA-1
• LFA1A
• LFA-1A
• Ly-15
• Ly-21
• lymphocyte function-associated antigen 1

Background

CD11a is a member of the integrin family of cell adhesion molecules. It is a glycoprotein expressed in combination with the CD18 beta chain. CD11a is a member of the beta 2 integrin family. These molecules function in cell adhesion and specifically bind to CD54, ICAM2, ICAM3. CD11a is expressed on thymocytes, T and B lymphocytes, granulocytes, monocytes, and macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGAL