Integrin alpha L/CD11a Antibody (DF1524) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: Flow, ICC/IF, ICC/IF
• Clone: DF1524
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

Integrin alpha L/CD11a Antibody (DF1524) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: Human Integrin alpha L/CD11a protein (Uniprot: P20701)
• Localization: Cell Surface
• Specificity: Recognizes a protein of 180kDa, identified as CD11a (Leucocyte Workshop IV; Code 1524). CD11a complex with the 2 subunit of the integrin family, CD18, to form the cell surface heterodimer, LFA-1 or CD11a /C18 (aLbL). LFA-1 is expressed on all leukocytes including lymphocytes, monocytes, and granulocytes. It is involved in leukocyte adhesion to its ligands including intercellular adhesion molecule-1 (ICAM-1 or CD54), ICAM-2 (CD102), ICAM-3 (CD50) and Telencephalin (TLN) and play a role in most immune/inflammatory responses. This monoclonal antibody potently blocks LFA-1 dependent homotypic cell aggregation.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGAL
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Integrin alpha L/CD11a Antibody (DF1524) [CoraFluor™ 1]

• CD11 antigen-like family member A
• CD11a antigen
• CD11a
• CD11Aantigen CD11A (p180), lymphocyte function-associated antigen 1, alphapolypeptide
• Integrin alpha L
• integrin alpha-L
• integrin gene promoter
• integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen1; alpha polypeptide)
• ITGAL
• Leukocyte adhesion glycoprotein LFA-1 alpha chain
• Leukocyte function-associated molecule 1 alpha chain
• LFA-1 alpha
• LFA-1
• LFA1A
• LFA-1A
• Ly-15
• Ly-21
• lymphocyte function-associated antigen 1

Background

CD11a is a member of the integrin family of cell adhesion molecules. It is a glycoprotein expressed in combination with the CD18 beta chain. CD11a is a member of the beta 2 integrin family. These molecules function in cell adhesion and specifically bind to CD54, ICAM2, ICAM3. CD11a is expressed on thymocytes, T and B lymphocytes, granulocytes, monocytes, and macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGAL