Integrin alpha E2/Dendritic Cells Antibody (OX-62)

Images

 
Flow Cytometry: Integrin alpha E2/Dendritic Cells Antibody (OX-62) [NB100-65618] - Rat splenocytes stained with phycoerythrin conjugated Mouse anti Rat OX-62 in red filled, with PE conjugated Mouse IgG1 isotype control ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, IP
Clone
OX-62
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Integrin alpha E2/Dendritic Cells Antibody (OX-62) Summary

Immunogen
Density gradient enriched PVG rat thoracic duct dendritic cells.
Specificity
NB100-65618 recognizes the OX-62 antigen, shown to be rat alpha E2 integrin which is expressed by dendritic cells of dendritic epidermal T cells, intraepithelial lymphocytes in the small intestine and by cells of denritic morphology in lymphoid organs, at sites where gamma delta T cells are present.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITGAE
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:25-1:100
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Integrin alpha E2/Dendritic Cells Antibody (OX-62)

  • antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide
  • CD103 antigen
  • CD103
  • HML-1 antigen
  • HUMINAE
  • integrin alpha-E
  • Integrin alpha-IEL
  • integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alphapolypeptide)
  • MGC141996
  • Mucosal lymphocyte 1 antigen

Background

Dendritic cells are potent antigen presenting cells that possess the ability to stimulate naive T cells. They are critical for the induction of T cell responses resulting in cell mediated immunity. Within different tissues, dendritic cells differentiate and become active in the taking up and processing of antigens, and their subsequent presentation on the cell surface linked to major histocompatibility (MHC) molecules. Upon appropriate stimulation, dendritic cells undergo further maturation and migrate to secondary lymphoid tissues where they present Ag to T cells and induce an immune response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NB100-65618AF405
Alexa Fluor 488 NB100-65618AF488
Alexa Fluor 647 NB100-65618AF647
Alexa Fluor 700 NB100-65618AF700
Allophycocyanin NB100-65618APC
DyLight 350 NB100-65618UV
DyLight 405 NB100-65618V
DyLight 488 NB100-65618G
DyLight 680 NB100-65618FR
DyLight 755 NB100-65618IR
PE NB100-65618PE
PerCP NB100-65618PCP

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Bioinformatics

Gene Symbol ITGAE
Uniprot