Integrin alpha E2/Dendritic Cells Antibody (OX-62) - BSA Free

Flow Cytometry: Integrin alpha E2/Dendritic Cells Antibody (OX-62) [NB100-65618] - Rat splenocytes stained with phycoerythrin conjugated Mouse anti Rat OX-62 in red filled, with PE conjugated Mouse IgG1 isotype control blue line.

• Reactivity: Rt
• Applications: WB, Flow, IHC, IHC-Fr, IP
• Clone: OX-62
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Integrin alpha E2/Dendritic Cells Antibody (OX-62) - BSA Free Summary

• Immunogen: Density gradient enriched PVG rat thoracic duct dendritic cells.
• Specificity: NB100-65618 recognizes the OX-62 antigen, shown to be rat alpha E2 integrin which is expressed by dendritic cells of dendritic epidermal T cells, intraepithelial lymphocytes in the small intestine and by cells of denritic morphology in lymphoid organs, at sites where gamma delta T cells are present.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGAE
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:25-1:100
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Integrin alpha E2/Dendritic Cells Antibody (OX-62) - BSA Free

• antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide
• CD103 antigen
• CD103
• HML-1 antigen
• HUMINAE
• integrin alpha-E
• Integrin alpha-IEL
• integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alphapolypeptide)
• MGC141996
• Mucosal lymphocyte 1 antigen

Background

Dendritic cells are potent antigen presenting cells that possess the ability to stimulate naive T cells. They are critical for the induction of T cell responses resulting in cell mediated immunity. Within different tissues, dendritic cells differentiate and become active in the taking up and processing of antigens, and their subsequent presentation on the cell surface linked to major histocompatibility (MHC) molecules. Upon appropriate stimulation, dendritic cells undergo further maturation and migrate to secondary lymphoid tissues where they present Ag to T cells and induce an immune response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGAE
• Uniprot:
  • O88341(Rat)