AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins. " alt="Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Goat Anti-Mouse Integrin alpha E/CD103 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1990) or (B) isotype control antibody (Catalog # AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB1990G | |||
| Carrier Free | AF1990-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97564-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88142-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1990-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65272-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-16987-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03545-20ul | |||
![Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free [NBP1-97564] - Separation of human CD103 positive cells (red-filled) from CD103 negative cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using anti-human CD103 (Ber-ACT8) purified antibody (concentration in sample 3 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/Integrin-alpha-E-CD103-Antibody-Ber-ACT8-BSA-Free-Flow-Cytometry-NBP1-97564-img0005.jpg "Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free [NBP1-97564] - Separation of human CD103 positive cells (red-filled) from CD103 negative cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using anti-human CD103 (Ber-ACT8) purified antibody (concentration in sample 3 ug/ml, GAM APC).")
![Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) [NBP1-97564] - Staining of CD103 on human peripheral blood cells with anti-CD103 (Ber-ACT8) APC.](http://images.novusbio.com/fullsize/Integrin-alpha-E-CD103-Antibody-Ber-ACT8-Flow-Cytometry-NBP1-97564-img0001.jpg)
![Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) [NBP1-97564] - Staining of CD103 on PHA-activated PBMC with anti-CD103 (Ber-ACT8) FITC.](http://images.novusbio.com/fullsize/Integrin-alpha-E-CD103-Antibody-Ber-ACT8-Flow-Cytometry-NBP1-97564-img0002.jpg)
![Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) [NBP1-97564] - Staining of CD103 on PHA-activated PBMC with anti-CD103 (Ber-ACT8) PE.](http://images.novusbio.com/fullsize/Integrin-alpha-E-CD103-Antibody-Ber-ACT8-Flow-Cytometry-NBP1-97564-img0003.jpg)
![Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free [NBP1-97564] - Staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD103 (Ber-ACT8) purified antibody (concentration in sample 3 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/Integrin-alpha-E-CD103-Antibody-Ber-ACT8-BSA-Free-Flow-Cytometry-NBP1-97564-img0004.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha E/CD103 Antibody [NBP1-88142] - Staining of human small intestine shows strong membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Integrin-alpha-E-CD103-Antibody-Immunohistochemistry-Paraffin-NBP1-88142-img0004.jpg "Immunohistochemistry-Paraffin: Integrin alpha E/CD103 Antibody [NBP1-88142] - Staining of human small intestine shows strong membranous positivity in lymphoid cells.")
![Immunohistochemistry-Paraffin: Integrin alpha E/CD103 Antibody [NBP1-88142] - Staining of human testis shows moderate to strong membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Integrin-alpha-E-CD103-Antibody-Immunohistochemistry-Paraffin-NBP1-88142-img0001.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha E/CD103 Antibody [NBP1-88142] - Staining of human endometrium shows no positivity in glandular cells.](http://images.novusbio.com/fullsize/Integrin-alpha-E-CD103-Antibody-Immunohistochemistry-Paraffin-NBP1-88142-img0002.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha E/CD103 Antibody [NBP1-88142] - Staining of human duodenum shows strong membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Integrin-alpha-E-CD103-Antibody-Immunohistochemistry-Paraffin-NBP1-88142-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin alpha E/CD103 Antibody [NBP3-16987] - Analysis in human small intestine and cerebral cortex tissues using Anti-ITGAE antibody. Corresponding ITGAE RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Integrin-alpha-E-CD103-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-16987-img0001.jpg "Immunocytochemistry/Immunofluorescence: Integrin alpha E/CD103 Antibody [NBP3-16987] - Analysis in human small intestine and cerebral cortex tissues using Anti-ITGAE antibody. Corresponding ITGAE RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: Integrin alpha E/CD103 Antibody [NBP3-16987] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/Integrin-alpha-E-CD103-Antibody-Immunohistochemistry-Paraffin-NBP3-16987-img0002.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha E/CD103 Antibody [NBP3-16987] - Staining of human small intestine shows high expression.](http://images.novusbio.com/fullsize/Integrin-alpha-E-CD103-Antibody-Immunohistochemistry-Paraffin-NBP3-16987-img0003.jpg)
![Immunoprecipitation: Integrin alpha E2/Dendritic Cells Antibody [NBP3-14689] - : Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from MJ cells preparedusing NETN lysis buffer. Antibodies: Rabbit antiCD103/ITGAE recombinant monoclonal antibody used for IP at 6 ul/mg lysate.CD103/ITGAE was also immunoprecipitated by a secondantibody against a different epitope of CD103/ITGAE. For blotting immunoprecipitatedCD103/ITGAE,was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Integrin-alpha-E2-Dendritic-Cells-Antibody-Immunoprecipitation-NBP3-14689-img0001.jpg "Immunoprecipitation: Integrin alpha E2/Dendritic Cells Antibody [NBP3-14689] - : Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from MJ cells preparedusing NETN lysis buffer. Antibodies: Rabbit antiCD103/ITGAE recombinant monoclonal antibody used for IP at 6 ul/mg lysate.CD103/ITGAE was also immunoprecipitated by a secondantibody against a different epitope of CD103/ITGAE. For blotting immunoprecipitatedCD103/ITGAE,was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: Integrin alpha E/CD103 Antibody [NBP3-03545] -Analysis of various lysates using ITGAE Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/antibody/nbp3-03545_rabbit-polyclonal-integrin-alpha-e-cd103-antibody-western-blot-6520258231..png "Western Blot: Integrin alpha E/CD103 Antibody [NBP3-03545] -Analysis of various lysates using ITGAE Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.")
![Flow Cytometry: Integrin alpha E2/Dendritic Cells Antibody (OX-62) [NB100-65618] - Rat splenocytes stained with phycoerythrin conjugated Mouse anti Rat OX-62 in red filled, with PE conjugated Mouse IgG1 isotype control blue line.](http://images.novusbio.com/fullsize/Integrin-alpha-E2-Dendritic-Cells-Antibody-OX-62-Flow-Cytometry-NB100-65618-img0001.jpg "Flow Cytometry: Integrin alpha E2/Dendritic Cells Antibody (OX-62) [NB100-65618] - Rat splenocytes stained with phycoerythrin conjugated Mouse anti Rat OX-62 in red filled, with PE conjugated Mouse IgG1 isotype control blue line.")
![Immunohistochemistry-Paraffin: Integrin alpha E/CD103 Antibody (SN62-06) [NBP2-67663] - Analysis of paraffin-embedded human small intestine tissue using anti-Integrin alpha E antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Integrin-alpha-E-CD103-Antibody-SN62-06-Immunohistochemistry-Paraffin-NBP2-67663-img0003.jpg "<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha E/CD103 Antibody (SN62-06) [NBP2-67663] - Analysis of paraffin-embedded human small intestine tissue using anti-Integrin alpha E antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha E/CD103 Antibody (SN62-06) [NBP2-67663] - Analysis of paraffin-embedded human tonsil tissue using anti-Integrin alpha E antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-E-CD103-Antibody-SN62-06-Immunohistochemistry-Paraffin-NBP2-67663-img0001.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha E/CD103 Antibody (SN62-06) [NBP2-67663] - Analysis of paraffin-embedded human lymph node tissue using anti-Integrin alpha E/CD103 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Integrin-alpha-E-CD103-Antibody-SN62-06-Immunohistochemistry-Paraffin-NBP2-67663-img0002.jpg)
