Integrin alpha 6 beta 1 Antibody (2548B) [Unconjugated]

Human PBMC were stained with (A) Rabbit Anti-Human Integrin a6 beta 1 Monoclonal Antibody (Catalog # MAB7809) or (B) Rabbit IgG control antibody (Catalog # MAB1050) followed by PE-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110) and Mouse anti-Human CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB100A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 2548B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Integrin alpha 6 beta 1 Antibody (2548B) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Chinese Hamster Ovary cell line, CHO-derived Human Integrin alpha 6 beta 1 heterodimer
  Phe24-Ser1012(Integrin alpha 6) and Gln21-Asp728 (Integrin beta1)
  Accession # NP_000201
• Specificity: Detects human Integrin alpha 6 beta 1 in direct ELISAs. In direct ELISA, less than 1% of cross reactivity with recombinant human (rh) Integrin beta 1 and recombinant mouse (rm) Integrin alpha 6 is observed. In direct ELISA, no cross-reactivity with rhIntegrin alpha 3, beta 2, beta 3, beta 5, beta 6, beta 7, and rmIntegrin beta 1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 6 beta 1 Antibody (2548B) [Unconjugated]

• CD49f
• Integrin alpha 6 beta 1
• ITGA6B
• VLA-6

Background

Integrin alpha 6 beta 1, also called platelet glycoprotein GPIc-IIa, is a laminin binding integrin that is expressed on T cells, monocytes, endothelial cells, stem cells, and platelets (1-9). The non-covalent heterodimer is composed of ~150 kDa alpha 6/CD49f and 130 kDa beta 1/CD29 type I transmembrane glycoprotein subunits (2). While alpha 6 pairs only with beta 1 or beta 4, twelve integrins share the beta 1 subunit (1-5). The alpha 6 subunit is cleaved into extracellular heavy and transmembrane light chains (3). Alternative splicing in the human alpha 6 extracellular domain (ECD) at amino acid (aa) 216 creates X1 (ubiquitous), X2 and X1X2 isoforms, while splicing at a mouse or human cytoplasmic site creates A and B isoforms (10, 11). These forms do not appear to alter the binding specificity (4, 10, 11). The beta 1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside-out) signaling convert it to its most active, extended and open conformation (1, 2). The human alpha 6 (X1) heavy chain shares 94‑95% aa identity with mouse, rat, bovine, and canine alpha 6, and the human beta 1 ECD shares 92‑96% aa sequence identity with rat, bovine, mouse, and feline beta 1. alpha 6 beta 1 shows broad specificity for adhesion to laminin isoforms (4, 10). Its expression on human and mouse pluripotent stem cells is important for attachment, expansion, and self‑renewal on LN‑511 (laminin  alpha ₅  beta ₁ gamma ₁) (6, 7). The secreted protein Netrin-4 and the laminin  gamma ₁ subunit form an adhesion‑activating complex with alpha 6 beta 1 on mouse neural stem cells and human lymphatic endothelial cells that promotes lymphangiogenesis (8, 9). alpha 6 beta 1 up‑regulation on cancers such as prostate, glioma, and hepatoma is reported to enhance tumorigenicity, motility, invasion and metastasis (12‑14). alpha 6 beta 1 cleavage via uPA (urokinase-type plasminogen activator) facilitates tumorigenicity in prostate cancers, and interaction of hepatoma alpha 6 beta 1 with EMMPRIN/CD147 may also enhance tumorigenicity by inducing uPA and other metalloproteinases (12, 13).

1. Takada, Y. et al. (2007) Genome Biol. 8:215.
2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
3. Tamura, R.N. et al. (1990) J. Cell Biol. 111:1593.
4. Nishiuchi, R. et al. (2006) Matrix Biol. 25:189.
5. Sonnenberg, A. and C.J.T. Linders (1990) J. Cell Science 96:207.
6. Rodin, S. et al. (2010) Nat. Biotech. 28:611.
7. Domogatskaya A. et al. (2008) Stem Cells 26:2800.
8. Staquicini, F.I. et al. (2009) Proc. Natl. Acad. Sci. USA 106:2903.
9. Larrieu-Lahargue, F. et al. (2011) Circ. Res. 109:770.
10. Delwel, G. O. et al. (1995) Cell Adhes. Commun. 3:143.
11. Hogervorst, F. et al. (1993) J. Cell Biol. 121:179.
12. Sroka, I.C. et al. (2011) Mol. Cancer Res. 9:1319.
13. Dai, J.Y. et al. (2009) BMC Cancer 9:337.
14. Delamarre, E. et al. (2009) Am. J. Pathol. 175:844.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • NP_000201()