Integrin alpha 5/CD49e Overexpression Lysate (Native)

Images

 
Western Blot: Integrin alpha 5 Overexpression Lysate (Adult Normal) [NBL1-12065] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Integrin alpha 5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Integrin alpha 5/CD49e Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201151 and based on accession number NM_002205. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens integrin, alpha 5 (fibronectin receptor, alpha polypeptide) (ITGA5), mRNA.
Gene
ITGA5

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Integrin alpha 5/CD49e

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Integrin alpha 5/CD49e Overexpression Lysate (Native)

  • CD49 antigen-like family member E
  • CD49e antigen
  • CD49e
  • Fibronectin receptor subunit alpha
  • fibronectin receptor, alpha subunit
  • FNRAVLA5A
  • Integrin alpha 5
  • integrin alpha-5
  • Integrin alpha-F
  • integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
  • ITGA5
  • very late activation protein 5, alpha subunit
  • VLA-5 alpha
  • VLA-5

Background

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Integrin alpha 5/CD49e Lysate (NBL1-12065) (0)

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Bioinformatics

Gene Symbol ITGA5
Entrez