Integrin alpha 5/CD49e Antibody (235112)

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clone: 235112
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin alpha 5/CD49e Antibody (235112) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 5/CD49e
  Phe45-Asn999 (Gln68Glu)
  Accession # P11688
• Specificity: Detects mouse Integrin alpha 5/CD49e in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: ITGA5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 5/CD49e Antibody (235112)

• CD49 antigen-like family member E
• CD49e antigen
• CD49e
• Fibronectin receptor subunit alpha
• fibronectin receptor, alpha subunit
• FNRAVLA5A
• Integrin alpha 5
• integrin alpha-5
• Integrin alpha-F
• integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• ITGA5
• very late activation protein 5, alpha subunit
• VLA-5 alpha
• VLA-5

Background

Integrin alpha 5 subunit, also called CD49e, associates with the Integrin beta 1 subunit (CD29) to form the VLA-5 complex. It is expressed on thymocytes, mast cells, activated T lymphocytes, and some mouse cell lines. It functions as a receptor for fibronectin (1‑4).

1. Springer, T.A. et al. (1982) Immunol. Rev. 68:171.
2. Kinashi, T. and T.A. Springer (1994) Blood Cells 20:25.
3. Halvorson, M.J. et al. (1995) J. Immunol. 55:4567.
4. Uhlenkott, E.C. et al. (1996) Clin. Exp. Metastasis 14:125.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 5/CD49e Antibody (MAB1754)(1)

Publication using MAB1754

• Batra N, Riquelme M, Burra S, Jiang J 14-3-3theta facilitates plasma membrane delivery and function of mechanosensitive connexin 43 hemichannels. J Cell Sci, 2013-10-25;127(0):137-46. 2013-10-25 [PMID: 24163432] (ICC, Immunoprecipitation, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: ITGA5
• Uniprot:
  • P11688()