Integrin alpha 5/CD49e Antibody (10F6)

Western Blot: Integrin alpha 5/CD49e Antibody (10F6) [NBP2-37666] - Western blot analysis using ITGA5 mouse mAb against ITGA5-hIgGFc transfected HEK293 cell lysate.

Immunohistochemistry: Integrin alpha 5/CD49e Antibody (10F6) [NBP2-37666] - Immunohistochemical analysis of paraffin-embedded human Placenta tissues using ITGA5 mouse mAb

Flow Cytometry: Integrin alpha 5/CD49e Antibody (10F6) [NBP2-37666] - Flow cytometric analysis of Hela cells using ITGA5 mouse mAb (green) and negative control (purple).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC
• Clone: 10F6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Integrin alpha 5/CD49e Antibody (10F6) Summary

• Immunogen: Purified recombinant fragment of human Integrin alpha 5/CD49e expressed in E. Coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA5
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 114 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Integrin alpha 5/CD49e Antibody (10F6)

• CD49 antigen-like family member E
• CD49e antigen
• CD49e
• Fibronectin receptor subunit alpha
• fibronectin receptor, alpha subunit
• FNRAVLA5A
• Integrin alpha 5
• integrin alpha-5
• Integrin alpha-F
• integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• ITGA5
• very late activation protein 5, alpha subunit
• VLA-5 alpha
• VLA-5

Background

CD49e is a type I integral membrane glycoprotein, known as alpha5 integrin, VLA-5 alpha chain. It forms noncovalent heterodimer with integrin beta1 (CD29). CD49e contains two disulfide-linked subunits of 135 kDa and 24 kDa, and is mainly expressed on thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, and some myeloid leukemia cells. CD49e/CD29 complex binds to fibronection and neural adhesion molecule L1. It mediates monocytes migration, involves in the regulation of cells survival and apoptosis, and co-stimulates T cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGA5