Integrin alpha 2b/CD41 Antibody (HIP8) - BSA Free

Flow Cytometry: Integrin alpha 2b/CD41 Antibody (HIP8) [NB500-549] - Surface staining of human peripheral blood cells with anti-CD41a (HIP8) purified, GAM-APC.

Product Discontinued

Integrin alpha 2b/CD41 Antibody (HIP8) - BSA Free Summary

• Additional Information: Clone HIP8 was used by HLDA to establish CD designation.
• Immunogen: Full length native protein (purified) (Human).
• Specificity: The antibody HIP8 reacts with alpha (a) subunit of CD41 (heavy chain; 120 kDa). CD41 is mainly expressed on platelets and megakaryocytes. The antibody HIP8 blocks platelet aggregation and completely inhibits ADP-, epinephrine-, and collagen-induced platelet activation, and partially inhibits ristocetin- and thrombin-induced platelet activation. HIP8 is useful in the morphological and physiological studies of platelets and megakaryocytes. HLDA IV; WS Code P 38
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA2B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1-4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
• Application Notes: Flow Cytometry: This HIP8 antibody has been tested by flow cytometric analysis of human platelets. This can be used at less than or equal to 0.5 ug per 100 ul blood (per 106 cells). This antibody is CyTOF ready.
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Integrin alpha 2b/CD41 Antibody (HIP8) - BSA Free

• CD41 antigen
• CD41
• CD41BHPA3
• GP2B
• GP2Bintegrin alpha-IIb
• GPalpha IIb
• GPIIb
• GTA
• HPA3
• Integrin alpha 2b
• integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigenCD41)
• integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigenCD41B)
• ITGA2b
• ITGAB
• platelet fibrinogen receptor, alpha subunit
• Platelet membrane glycoprotein IIb
• platelet-specific antigen BAK

Background

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metatastatic diffusion of tumour cells. This protein is identified as capable of immunoprecipitating a non-reducible alpha subunit from I125 surface labelled Human cell line extract. It is confirmed as specific to the alpha 2 subunit by relative expression of antigen on various cell lines by Flow Cytometry, and its recognition of affinity purified alpha 2 beta 1 in dot blots.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGA2B