Insulin Receptor beta [p Thr1160] Antibody - Azide Free

Western Blot: Insulin Receptor Beta [p Thr1160] Antibody [NBP2-60777] - Western blot of HeLa cell lysate showing specific labeling of the~95kDa IR protein phosphorylated at Thr1160 in the first lane (-).Phosphospecificity is shown in the second lane (+) whereimmunolabeling is completely eliminated by blot treatment withlambda phosphatase (Lambda-Ptase, 1200 units for 30 min).

• Reactivity: Hu, Mu, Rt, Bv, Ca, Pm, Xp
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

Insulin Receptor beta [p Thr1160] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit Insulin Receptor beta [p Thr1160] Antibody - Azide Free (NBP2-60777) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr1160 conjugated to KLH. Accession # P06213
• Modification: p Thr1160
• Specificity: Specific for endogenous levels of the ~95 kDa Insulin receptor phosphorylated at Thr1160. Immunolabeling is completely eliminated with lambda-phosphatase treatment.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Primate (100%), Xenopus (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: INSR
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Insulin Receptor beta [p Thr1160] Antibody - Azide Free

• CD220 antigen
• CD220
• EC 2.7.10
• EC 2.7.10.1
• HHF5
• insulin receptor
• IR

Background

The Insulin Receptor (InsR) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. InsR is activated upon binding of the peptide hormone insulin, leading to autophosphorylation of tyrosine residues 1146, 1150, and 1151 in the activation loop of the beta-chain. Additional autophosphorylation sites such as tyrosine residues 960, 972, 1316, and 1322 regulate the assembly of signal transduction complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INSR