Importin-7 Antibody

Images

 
Independent Antibodies: Western Blot: Importin-7 Antibody [NBP1-46168] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. ...read more
Immunohistochemistry: Importin-7 Antibody [NBP1-46168] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Importin 7 used at a dilution of 1:1,000 (0.2ug/ml) Detection: DAB staining ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Importin-7 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 200 and 250 of human Importin 7 using the numbering given in entry NP_006382.1 (GeneID 10527).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IPO7
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 5-15 ug/mg lysate
Application Notes
For IHC-P: Epitope retrieval with citrate buffer pH 6.0 is recommended
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Importin-7 Antibody

  • FLJ14581
  • Imp7MGC138673
  • importin 7
  • RAN binding protein 7
  • Ran-binding protein 7
  • RanBP7
  • RANBP7importin-7

Background

The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. Similar to importin-beta, this protein prevents the activation of Ran's GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP, and also binds directly to nuclear pore complexes where it competes for binding sites with importin-beta and transportin. This protein has a Ran-dependent transport cycle and it can cross the nuclear envelope rapidly and in both directions. At least four importin beta-like transport receptors, namely importin beta itself, transportin, RanBP5 and RanBP7, directly bind and import ribosomal proteins. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IPO7
Entrez