Karyopherin (importin) beta 3 Antibody (1C4)

Western Blot: Karyopherin (importin) beta 3 Antibody (1C4) [H00003843-M01] - Analysis of IPO5 expression in transfected 293T cell line by RANBP5 monoclonal antibody (M01), clone 1C4.Lane 1: IPO5 transfected lysate (Predicted MW: 125.6 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: Karyopherin (importin) beta 3 Antibody (1C4) [H00003843-M01] - Analysis of monoclonal antibody to RANBP5 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.

Western Blot: Karyopherin (importin) beta 3 Antibody (1C4) [H00003843-M01] - RANBP5 monoclonal antibody (M01), clone 1C4 Analysis of RANBP5 expression in HeLa.

Western Blot: Karyopherin (importin) beta 3 Antibody (1C4) [H00003843-M01] - RANBP5 monoclonal antibody (M01), clone 1C4. Analysis of IPO5 expression in PC-12.

Western Blot: Karyopherin (importin) beta 3 Antibody (1C4) [H00003843-M01] - RANBP5 monoclonal antibody (M01), clone 1C4. Analysis of IPO5 expression in Raw 264.7.

ELISA: Karyopherin (importin) beta 3 Antibody (1C4) [H00003843-M01] - Detection limit for recombinant GST tagged RANBP5 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, IHC
• Clone: 1C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Karyopherin (importin) beta 3 Antibody (1C4) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RANBP5 (AAH01497, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAAAAEQQQFYLLLGNLLSPDNVVRKQAEETYENIPGQSKITFLLQAIRNTTAAEEARQMAAVLLRRLLSSAFDEVYPALPSDVQTAIKSELLMIIQME
• Localization: Cytoplasmic and Nuclear. Note=Found particularly in the nuclear rim and nucleolus.
• Specificity: RANBP5 - RAN binding protein 5
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IPO5
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Karyopherin (importin) beta 3 Antibody (1C4)

• DKFZp686O1576
• FLJ43041
• IMB3
• imp5
• importin 5
• importin beta-3 subunit
• Importin subunit beta-3
• importin-5
• karyopherin (importin) beta 3
• Karyopherin beta-3
• KPNB3
• MGC2068
• RAN binding protein 5
• Ran_GTP binding protein 5
• Ran-binding protein 5
• RANBP5

Background

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Karyopherin (importin) beta 3 Antibody (H00003843-M01)(2)

Publications using H00003843-M01

• Song S, Lee JJ, Kim HJ et al. Fas-Associated Factor 1 Negatively Regulates Antiviral Immune Response by Inhibiting Translocation of IRF3 to the Nucleus. Mol Cell Biol 2016-01-25 [PMID: 26811330]
• Hurd TW, Fan S, Margolis BL. Localization of retinitis pigmentosa 2 to cilia is regulated by Importin {beta}2. J Cell Sci. 2011-02-01 [PMID: 21285245]

Bioinformatics

• Gene Symbol: IPO5
• Entrez:
  • 3843(Human)
• OMIM:
  • 602008(Human)
• Uniprot:
  • AAH01497(Human)