IMP2/IGF2BP2 Antibody

Western Blot: IMP2/IGF2BP2 Antibody [23750002] - Samples: Lane 1, Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil Target weight [kDa]: 66, 62 (splice variants) Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.

Immunohistochemistry: IMP2/IGF2BP2 Antibody [23750002] - Both normal and malignant tissues showed moderate to strong cytoplasmic staining. In normal tissues, surface epithelial cells of bronchus, glandular cells of the intestine, endometrium and cervix, and trophoblastic cells displayed strong cytoplasmic staining. Pancreas, surface epithelial cells of oral mucosa, vagina and cervix, non-neuronal cells of CNS tissues and lymphoid tissues were negative. Colo-rectal, skin and testis carcinomas exhibited strong cytoplasmic staining while ovarian, stomach and pancreas malignancies displayed moderate to strong cytoplasmic staining. Malignant carcinoids showed weak cytoplasmic and nuclear staining. Renal and prostate malignancies were weak to negative. Image and statement courtesy of the Human Protein Atlas (HPA).

ELISA: IMP2/IGF2BP2 Antibody [23750002]

Product Discontinued

IMP2/IGF2BP2 Antibody Summary

• Immunogen: This antibody is specific for the N Terminus Region of the target protein.
• Specificity: This product is specific for Human IGF2BP2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IGF2BP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:250-1:2000
  • Western Blot 1:5000-1:20000
• Application Notes: This antibody is useful in ELISA, Immunohistochemistry-Paraffin and Western Blot.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for IMP2/IGF2BP2 Antibody

• Hepatocellular carcinoma autoantigen p62
• IF2B2
• IGF2BP2
• IGF-II mRNA-binding protein 2
• IMP2
• IMP-2IGF2 mRNA-binding protein 2
• IMP2VICKZ family member 2
• insulin-like growth factor 2 mRNA binding protein 2
• insulin-like growth factor 2 mRNA-binding protein 2
• p62
• VICKZ2

Background

This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains several four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IMP2/IGF2BP2 Antibody (23750002)(2)

Publications using 23750002

• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Bioinformatics

• Gene Symbol: IGF2BP2
• Uniprot:
  • Q9Y6M1()