Human IMP Dehydrogenase 2/IMPDH2 ELISA Kit (Colorimetric)

ELISA: Human IMP Dehydrogenase 2/IMPDH2 ELISA Kit (Colorimetric) [NBP3-39947] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Standard Curve Range: 0.781 - 50 ng/mL (example only; lot dependent)
• Sensitivity: 0.27 ng/mL (example only; lot dependent)

Human IMP Dehydrogenase 2/IMPDH2 ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 4.5 hours
• Standard Curve Range: 0.781 - 50 ng/mL (example only; lot dependent)
• Sensitivity: 0.27 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IMPDH2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human IMP Dehydrogenase 2/IMPDH2 ELISA Kit (Colorimetric)

• EC 1.1.1.205
• IMDH2
• IMP (inosine 5'-monophosphate) dehydrogenase 2
• IMP (inosine monophosphate) dehydrogenase 2
• IMP Dehydrogenase 2
• IMP Oxireductase 2
• IMPD 2
• IMPD2
• IMPD2IMP oxireductase 2
• IMPDH 2
• IMPDH2
• IMPDH-II
• inosine 5' phosphate dehydrogenase 2
• inosine monophosphate dehydrogenase type II
• inosine-5'-monophosphate dehydrogenase 2

Background

IMPDH2 encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for IMP Dehydrogenase 2/IMPDH2 ELISA Kit (NBP3-39947). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IMPDH2