IMP Dehydrogenase 2/IMPDH2 Antibody (JE40-87)

Western Blot: IMP Dehydrogenase 2/IMPDH2 Antibody (JE40-87) [NBP2-75541] - Analysis of IMPDH2 on Daudi cell lysate using anti-IMPDH2 antibody at 1/2,000 dilution.

Immunocytochemistry/Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody (JE40-87) [NBP2-75541] - Staining IMPDH2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: IMP Dehydrogenase 2/IMPDH2 Antibody (JE40-87) [NBP2-75541] - Analysis of paraffin-embedded rat kidney tissue using anti-IMPDH2 antibody. Counter stained with hematoxylin.

Flow Cytometry: IMP Dehydrogenase 2/IMPDH2 Antibody (JE40-87) [NBP2-75541] - Analysis of Daudi cells with IMPDH2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody (JE40-87) [NBP2-75541] - Staining IMPDH2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody (JE40-87) [NBP2-75541] - Staining IMPDH2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: IMP Dehydrogenase 2/IMPDH2 Antibody (JE40-87) [NBP2-75541] - Analysis of paraffin-embedded human tonsil tissue using anti-IMPDH2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: IMP Dehydrogenase 2/IMPDH2 Antibody (JE40-87) [NBP2-75541] - Analysis of paraffin-embedded human thyroid gland tissue using anti-IMPDH2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: IMP Dehydrogenase 2/IMPDH2 Antibody (JE40-87) [NBP2-75541] - Analysis of paraffin-embedded mouse testis tissue using anti-IMPDH2 antibody. Counter stained with hematoxylin.

Product Discontinued

IMP Dehydrogenase 2/IMPDH2 Antibody (JE40-87) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human IMP Dehydrogenase 2/IMPDH2 aa 80-320 / 514. (SwissProt: P12268 Human; SwissProt: P24547 Mouse; SwissProt: E9PU28 Rat)
• Localization: Nucleus. Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IMPDH2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:50
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IMP Dehydrogenase 2/IMPDH2 Antibody (JE40-87)

• EC 1.1.1.205
• IMDH2
• IMP (inosine 5'-monophosphate) dehydrogenase 2
• IMP (inosine monophosphate) dehydrogenase 2
• IMP Dehydrogenase 2
• IMP Oxireductase 2
• IMPD 2
• IMPD2
• IMPD2IMP oxireductase 2
• IMPDH 2
• IMPDH2
• IMPDH-II
• inosine 5' phosphate dehydrogenase 2
• inosine monophosphate dehydrogenase type II
• inosine-5'-monophosphate dehydrogenase 2

Background

IMPDH2 encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IMPDH2