ILT4/CD85d/LILRB2 Antibody (42D1) [PE]

Product Discontinued

ILT4/CD85d/LILRB2 Antibody (42D1) [PE] Summary

• Immunogen: The immunogen for this antibody was ILT4.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: LILRB2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: This 42D1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 uL (1 ug)/per test. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Reactivity Notes

This antibody is reactive to Human.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS [pH 7.2] containing BSA and/or gelatin for protein stabilization
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for ILT4/CD85d/LILRB2 Antibody (42D1) [PE]

• CD85 antigen-like family member D
• CD85d antigen
• CD85d
• Ig-like transcript 4
• ILT4
• ILT-4
• ILT4CD85d
• Immunoglobulin-like transcript 4
• Leukocyte immunoglobulin-like receptor 2
• leukocyte immunoglobulin-like receptor subfamily B member 2 soluble isoform
• leukocyte immunoglobulin-like receptor subfamily B member 2
• leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains)
• LILRB2
• LIR2
• LIR2CD85D
• LIR-2subfamily A (with TM domain), member 6
• member 2
• MIR10
• MIR10LILRA6
• Monocyte/macrophage immunoglobulin-like receptor 10

Background

The monoclonal antibody 42D1 recognizes the CD85d protein also known as ILT4, LIR-2. It is a member of the ILT (immunoglobulin-like transcript)/ LIR (leukocyte Ig-like receptor), MIR (monocytes Ig-like receptor) family. CD85d is a 110 kDa single transmembrane receptor with 3 Ig-like domains and a long cytoplasmic domain containing ITIMs (immunoreceptor tyrosine-based inhibitory motifs). Expression is confined to monocytes, macrophages and dendritic cells. The ligand for CD85d is non-classical class I HLA-G, -B and G1. Upon ligand binding, tyrosine phosphatase SHP-1 interacts with the ITIM. Interaction of HLA-G with ILT4/CD85d is thought to contribute to tolerance during fetal implantation. Additionally, it is the levels of ILT3 and ILT4 on DCs that suggests a role in organ allograft rejection. Crosslinking of the receptor with the monoclonal antibody 42D1 inhibits calcium flux as well as enhancing HLA-G tetramer staining and restoring the stimulating activity of tolerogenic DC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LILRB2