IL1RAPL2 Antibody [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

IL1RAPL2 Antibody [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL1RAPL2
  Thr17-Glu356
  Accession # Q9NP60
• Specificity: Detects human IL1RAPL2 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant mouse IL1RAPL2 is observed. In Western blots, approximately 5% cross‑reactivity with recombinant human (rh) IL‑1&nbs
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL1RAPL2 Antibody [Alexa Fluor® 647]

• IL-1R10
• IL-1R-9
• IL1R9IL-1 receptor accessory protein-like 2
• IL-1R9IL-1RAPL-2
• IL1RAPL2
• IL-1RAPL2
• IL-1-RAPL-2
• IL1RAPL-2IL1RAPL-2-related protein
• interleukin 1 receptor 9
• interleukin 1 receptor accessory protein-like 2
• Three immunoglobulin domain-containing IL-1 receptor-related 1
• TIGIRR-1
• TIGIRR-1Interleukin-1 receptor 9
• X-linked interleukin-1 receptor accessory protein-like 2

Background

The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL-1 R3), ST2 (T1/IL-1 R4), IL‑18 R alpha (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL‑18 R beta (AcPL/IL-1 R7), IL1RAPL1 (TIGIRR-2/IL-1 R8), and IL1RAPL2 (TIGIRR-1/IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL‑18BP, differ from the other members by having only one Ig domain (1). IL-1 receptor accessory protein-like 2 (IL-1 RAPL2) is alternately known as IL-1 R9 and three immunoglobulin domain containing IL-1 receptor-related molecule 1 (TIGIRR-1) and is expressed in the brain (2). Its sequence predicts an 686 amino acid (aa) residue type I transmembrane glycoprotein with a 17 aa signal peptide, a 339 aa extracellular region containing three Ig-like domains, an 18 aa transmembrane domain and a 312 aa cytoplasmic tail (3). By comparison to other IL-1 receptor family proteins, IL1RAPL2 has a C-terminal cytoplasmic extension beyond the TIR domain that is found in IL1RAPL1 and SIGIRR but not other family members (3). Human and mouse IL1RAPL2 share approximately 95% aa sequence identity. Human IL1RAPL2 is most homologous (63%) to IL1RAPL1, a receptor protein that is highly expressed in hippocampus and is involved in X-linked mental retardation (4, 5). Genes for both have been localized to human chromosome Xq22. A ligand for IL1RAPL2 has not been identified (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.