Human IL18R1 ELISA Kit (Colorimetric)

ELISA: Human IL18R1 ELISA Kit (Colorimetric) [NBP2-80338] - Example standard curve for Human IL18R1 ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP
• Suitable Sample Type: Serum
• Standard Curve Range: 7.81-500 pg/ml (example only; lot dependent)
• Sensitivity: 3.49 pg/ml

Human IL18R1 ELISA Kit (Colorimetric) Summary

• Specificity: Recognizes both recombinant and natural Human IL18R1.
• Standard Curve Range: 7.81-500 pg/ml (example only; lot dependent)
• Sensitivity: 3.49 pg/ml
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 10.9 %
• Intra-Assay: CV% < 4.6
• Spike Recovery: 83 -90%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL18R1

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage is content dependent.

Alternate Names for Human IL18R1 ELISA Kit (Colorimetric)

• CD218 antigen-like family member A
• CD218a antigen
• CD218a
• CDw218a
• cytokine receptor
• IL-18R1
• IL-18R-1
• IL1R-rp
• IL1RRPIL1 receptor-related protein
• IL-1RrpIL18RA
• interleukin 18 receptor 1
• interleukin-18 receptor 1

Background

IL-18 receptor is composed of an alpha and a beta subunit that combine to form a high affinity receptor for IL-18. IL-18 receptor alpha chain, also known as CDw218a, is a 75-80 kD type I transmembrane protein. It is expressed on NK cells, neutrophils, endothelial cells, and subsets of T and B cells. The expression of CDw218a on lymphocytes is upregulated after activation. The interaction of IL-18 and IL-18 receptor has been reported to be implicated in promotion of Th1 cytokine production and atherogenesis. The H44 antibody is specific for IL-18 receptor alpha chain and has been reported to block IL-18 binding to the receptor and to be useful for the identification of IL-18 receptor by flow cytometry and IHC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for IL18R1 ELISA Kit (NBP2-80338). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL18R1