IL-6R alpha Antibody (Hu137) [Unconjugated]

HEK293 Human cell line transfected with (A) Human IL-6R alpha or (B) irrelevant protein, and eGFP was stained with Human Anti-Human IL-6R alpha (Research Grade Sarilumab Biosimilar) Monoclonal Antibody (Catalog # MAB10346) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

U937 human histiocytic lymphoma cell line was stained with Human Anti-Human IL-6R alpha (Research Grade Sarilumab Biosimilar) Monoclonal Antibody (Catalog # MAB10346, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Recombinant Human IL-6 R alpha (Catalog # 227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant Human IL-6 R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Human Anti-Human IL-6 R alpha (Research Grade Sarilumab) Monoclonal Antibody (Catalog # MAB10346). The ND50 is typically 0.04-0.2 µg/mL.

Biosimilar antibody development at Bio-Techne includes in silico design and engineering to generate both light chain (lc) and heavy chain (hc) sequences for each antibody. After transfection of the dual expression vector, Bio-Techne’s biosimilar antibodies undergo rigorous application testing to ensure the final biosimilar product is suited for your research needs.

• Reactivity: Hu
• Applications: Flow, B/N, CyTOF-ready
• Clone: Hu137
• Clonality: Monoclonal
• Host: Human
• Conjugate: Unconjugated

IL-6R alpha Antibody (Hu137) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody For Research Use Only
• Immunogen: Human IL-6R alpha
• Specificity: Detects Human IL-6R alpha based on Sarilumab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Sarilumab. This product is for research use only.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Human
• Gene: IL6R
• Purity Statement: Protein A or G purified from cell culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization 0.04-0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-6R alpha Antibody (Hu137) [Unconjugated]

• CD126
• gp80
• IL-6 R alpha
• IL6Q
• IL6R alpha
• IL-6R alpha
• IL6R
• IL-6R-1
• IL6RA
• IL-6Ra
• IL6RQ
• interleukin 6 receptor

Background

Sarilumab is a biosimilar directed against human IL-6R alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL6R