IL-6 Luciferase - (LUCPorter™) Stable Reporter Cell Line

Product Discontinued

IL-6 Luciferase - (LUCPorter™) Stable Reporter Cell Line Summary

• Description: The IL-6/LUCPoter(TM) reporter cell line is designed to monitor the induction of IL-6 and can be used for screening of agonists, antagonists or signaling inhibitors of IL-6 induction as well as for studying the IL-6 induction-related signaling pathways.
  
  Contents: 3~4 x 10^6 cells
  Biosafety Level: 1
• Immunogen: The IL-6/LUCPorter(TM) reporter cell line is a stably transfected NIH-3T3 cell line which expresses an optimized Renilla luciferase reporter gene (RenSP) under the transcriptional control of the IL-6 promoter. As a pleiotropic cytokine, interleukin 6 (IL-6) has pro- and anti-inflammatory roles which is not only involved in normal functions of the immune system, hematopoiesis and metabolism but also involved in the pathogenesis of metabolic and cardiovascular diseases. IL-6 gene induction is generally regulated by several transcription factors that activate the consensus sequences in the IL-6 promoter region, which include AP-1, C/EBP-beta and NF-kB in response to various proinflammatory cytokines, growth factors, and pathogen-associated molecular patterns such as TLR ligands. As shown in Figure 1, the IL-6 promoter activity in the IL-6/LUCPorter(TM) reporter cell line was induced by LPS in a dose response manner (Figure 1).
• Selection Agent: Puromycin at 3 ug/ml
• Growth Properties: Adherent
  Morphology : Fibroblast
• RCL Type: Luciferase - (LUCPorter™)
• Host: NIH-3T3
• Gene: IL6

Applications/Dilutions

• Dilutions:
  • Ligand Activation
• Readout System:
  • LightSwitch Luciferase Assay Reagent NBP2-32066

Packaging, Storage & Formulations

• Storage: Store in gas phase of liquid nitrogen.
• Reconstitution Instructions: Complete Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin (Note: The selection agents for this cell line is puromycin at 3 ug/ml).

Alternate Names for IL-6 Luciferase - (LUCPorter™) Stable Reporter Cell Line

• B-cell differentiation factor
• B-cell stimulatory factor 2
• BSF2
• BSF-2
• CDF
• CTL differentiation factor
• HSF
• hybridoma growth factor
• IFNB2
• IFN-beta-2
• IL6
• IL-6
• Interferon beta-2
• interleukin 6 (interferon, beta 2)
• interleukin BSF-2
• interleukin-6
• MGI-2A

Background

IL-6 is a multifunctional cytokine that has an important role in host defense by regulating immune and inflammatory responses. It is expressed by a variety of normal and transformed cells including T cells, B cells, monocytes/macrophages, fibroblasts and various tumor cells. IL-6 has diverse biological functions, it stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. Recombinant human IL-6 is a 20.9 kDa protein containing 184 amino acid residues. Human IL-6 is active on murine cells but murine IL-6 is inactive on human cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.

FAQs for IL-6 Reporter Cell Line (NBP2-32793). (Showing 1 - 1 of 1 FAQ).

1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
   • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

Bioinformatics

• Gene Symbol: IL6