Recombinant Human IL-6 Protein (NBP2-34901)

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SDS-Page: Human IL-6 Protein [NBP2-34901]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Concentration
LYOPH

Order Details

Recombinant Human IL-6 Protein Summary

Description
A single, non-glycosylated biologically active polypeptide chain corresponding to 183 residues of IL6.

Source: E. coli

Amino Acid Sequence: VPPGEDSKDV AAPHRQPLTS SERIDKQIRY ILDGISALRK ETCNKSNMCE SSKEALAENN LNLPKMAEKD GCFQSGFNEE TCLVKIITGL LEFEVYLEYL QNRFESSEEQ ARAVQMSTKV LIQFLQKKAK NLDAITTPDP TTNASLLTKL QAQNQWLQDM TTHLILRSFK EFLQSSLRAL RQM

Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Details of Functionality
IL6 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x 10^7 IU/mg.
Protein/Peptide Type
Biologically Active Protein
Gene
IL6
Purity
>95% pure by SDS-PAGE
Endotoxin Note
Less than 1 EU/ug of endotoxin as determined by LAL method.

Applications/Dilutions

Theoretical MW
20.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Concentration
LYOPH
Purity
>95% pure by SDS-PAGE
Reconstitution Instructions
Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Human IL-6 Protein

  • B cell stimulatory factor-2
  • B-cell differentiation factor
  • BSF-2
  • BSF2CTL differentiation factor
  • CDF
  • HGFHSFIFNB2Hybridoma growth factor
  • IFN-beta-2
  • IL6
  • IL-6
  • IL-6B-cell stimulatory factor 2
  • Interferon beta-2
  • interleukin 6 (interferon, beta 2)
  • interleukin BSF-2
  • interleukin-6
  • MGI-2A

Background

Interleukin-6 (IL-6) is an interleukin that in humans is encoded by the IL-6 gene and acts as both a pro-inflammatory and antiinflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response. Furthermore, It plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It also induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. The human IL-6 is a single non-glycosylated polypeptide chain containing 183 amino acids and it signals through a cell-surface type I cytokine receptor complex consisting of the ligand-binding IL-6Ralpha chain (CD126), and the signal- transducing component gp130 (also called CD130). The human IL-6 shares about 40 % a.a. sequence identity with mouse and rat IL-6 and it is equally active on mouse and rat cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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FAQs for IL-6 Protein (NBP2-34901). (Showing 1 - 1 of 1 FAQ).

  1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
    • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

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Bioinformatics

Gene Symbol IL6
Entrez
Uniprot