Rat IL-6 ELISA Kit (Colorimetric)

ELISA: IL-6 ELISA Kit [Biotin] [NBP1-92697] - Standard curve

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum
• Standard Curve Range: 31.0 - 2,000 pg/mL
• Sensitivity: 12.0 pg/mL

Rat IL-6 ELISA Kit (Colorimetric) Summary

• Description: The rat IL-6 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of rat IL-6
• Specificity: The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a rat IL-6 positive serum. There was no crossreactivity detected.
• Standard Curve Range: 31.0 - 2,000 pg/mL
• Sensitivity: 12.0 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 10%
• Intra-Assay: CV% < 5.0%
• Spike Recovery: Please See Protocol for Specific Spike Recovery Details
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL6

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.
• Buffer: Please see components for buffer contents.

Alternate Names for Rat IL-6 ELISA Kit (Colorimetric)

• B-cell differentiation factor
• B-cell stimulatory factor 2
• BSF2
• BSF-2
• CDF
• CTL differentiation factor
• HSF
• hybridoma growth factor
• IFNB2
• IFN-beta-2
• IL6
• IL-6
• Interferon beta-2
• interleukin 6 (interferon, beta 2)
• interleukin BSF-2
• interleukin-6
• MGI-2A

Background

Interleukin-6 (IL-6) is a multi-functional cytokine that regulates immune responses, acute phase reactions and hematopoiesis and may play a central role in host defense mechanisms. IL-6 is usually not produced constitutively by normal cells, but its expression is readily induced by a variety of cytokines, lipopolysaccharide or viral infections. IL-6 is a pleiotropic cytokine produced by a variety of cells. It acts on a wide range of tissues, exerting growth-induction, growth-inhibition, and differentiation respectively, depending on the nature of the target cells. IL-6 is involved in - the induction of B-cell differentiation - the induction of acute phase proteins in liver cells - growth promotion of myeloma/plasmacytoma/hybridoma cells - induction of IL-2 and IL-2 receptor expression - proliferation and differentiation of T cells - inhibition of cell growth of certain myeloid leukemic cell lines and induction of their differentiation to macrophages - enhancement of IL-3-induced multipotential colony cell formation in hematopoietic stem cells and induction of maturation of megakaryocytes as a thrombopoietic factor - induction of mesangial cell growth - induction of neural differentiation of PC 12 cells and - induction of keratinocyte growth The abnormal production of IL-6 was first suggested to be related to polyclonal B-cell activation with autoantibody production in patients with cardiac myxoma. Since then, IL-6 has been suggested to be involved in the pathogenesis of a variety of diseases. Measurement of IL-6 levels in serum and other body fluids thus provides more detailed insights into various pathological situations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for IL-6 ELISA Kit (NBP1-92697)(3)

Publications using NBP1-92697

• Mohamed K, Abdelfattah M, El-khadragy M et al. Rutin-loaded selenium nanoparticles modulated the redox status, inflammatory, and apoptotic pathways associated with pentylenetetrazole-induced epilepsy in mice Green Processing and Synthesis 2023-01-26 (ELISA, Mouse)
• ALRashdi BM, Mohamed RA, Mohamed AH et al. Therapeutic activity of green synthesized selenium nanoparticles from turmeric against cisplatin-induced oxido-inflammatory stress and cell death in mice kidney Bioscience reports 2023-11-30 [PMID: 37902021]
• Liu Y, Shen W, Chen Q et al. Inhibition of RAGE by FPS-ZM1 alleviates renal injury in spontaneously hypertensive rats European Journal of Pharmacology 2020-06-01 [PMID: 32502492] (ELISA, Rat)

FAQs for IL-6 ELISA Kit (NBP1-92697). (Showing 1 - 1 of 1 FAQs).

1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
   • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

Bioinformatics

• Gene Symbol: IL6