IL-6 Antibody [Unconjugated]

Recombinant Porcine IL‑6 (Catalog # 686-PI) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑6 (12.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF686). The ND50 is typically 0.15-0.6 µg/mL.

• Reactivity: Po
• Applications: WB, B/N, ELISA(Cap)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-6 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant porcine IL-6
  Pro29-Met212
  Accession # P26893
• Specificity: Detects porcine IL-6 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant human IL‑6, recombinant mouse IL‑6, and recombinant rat IL‑6 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL6
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.15-0.6 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Porcine IL-6 Antibody (Catalog # AF686)
  ELISA Detection: Porcine IL-6 Biotinylated Antibody (Catalog # BAF686)
  Standard: Recombinant Porcine IL-6 (Catalog # 686-PI)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-6 Antibody [Unconjugated]

• B-cell differentiation factor
• B-cell stimulatory factor 2
• BSF2
• BSF-2
• CDF
• CTL differentiation factor
• HSF
• hybridoma growth factor
• IFNB2
• IFN-beta-2
• IL6
• IL-6
• Interferon beta-2
• interleukin 6 (interferon, beta 2)
• interleukin BSF-2
• interleukin-6
• MGI-2A

Background

Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28 kDa phosphorylated and variably glycosylated molecule (1 - 4). Mature porcine IL-6 is 183 amino acids (aa) in length and shares 61%, 42%, and 42% aa sequence identity with human, mouse, and rat IL-6 (5). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (6). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (7). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL‑6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 8).

1. Van Snick, J. (1990) Annu. Rev. Immunol. 8:253.
2. Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502.
3. Jones, S.A. (2005) J. Immunol. 175:3468.
4. Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227.
5. Mathialagan, N. et al. (1992) Mol. Reprod. Dev. 32:324.
6. Murakami, M. et al. (1993) Science 260:1808.
7. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
8. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-6 Antibody (AF686)(9)

Publications using AF686

• SA Hall, SH Ison, C Owles, J Coe, DA Sandercock, AJ Zanella Development and validation of a multiplex fluorescent microsphere immunoassay assay for detection of porcine cytokines MethodsX, 2019-05-17;6(0):1218-1227. 2019-05-17 [PMID: 31193967] (ELISA Capture, Porcine)
• H Lv, W Dong, Z Cao, X Li, J Wang, G Qian, Q Lv, C Wang, K Guo, Y Zhang TRAF6 is a novel NS3-interacting protein that inhibits classical swine fever virus replication Sci Rep, 2017-07-27;7(1):6737. 2017-07-27 [PMID: 28751780] (ELISA Development (Capture), Porcine)
• S Elahi, DR Thompson, J Van Kessel, LA Babiuk, V Gerdts Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets Infect. Immun., 2017-03-23;85(4):. 2017-03-23 [PMID: 28167667] (ELISA Development (Capture), Porcine)
• Yang YJ, Qian HY, Huang J, Geng YJ, Gao RL, Dou KF, Yang GS, Li JJ, Shen R, He ZX, Lu MJ, Zhao SH Atorvastatin treatment improves survival and effects of implanted mesenchymal stem cells in post-infarct swine hearts. Eur. Heart J., 2008-05-02;29(12):1578-90. 2008-05-02 [PMID: 18456710] (Western Blot, Porcine)
• Saurer L, McCullough KC, Summerfield A In vitro induction of mucosa-type dendritic cells by all-trans retinoic acid. J. Immunol., 2007-09-15;179(6):3504-14. 2007-09-15 [PMID: 17785784] (Neutralization, Porcine)
• Nielsen JS, Larsson A, Rix T, Nyboe R, Gjedsted J, Krog J, Ledet T, Tonnesen E The effect of activated protein C on plasma cytokine levels in a porcine model of acute endotoxemia. Intensive Care Med, 2007-04-25;33(6):1085-93. 2007-04-25 [PMID: 17457574] (ELISA Development, Human)
• Azevedo MS, Yuan L, Pouly S, Gonzales AM, Jeong KI, Nguyen TV, Saif LJ Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus. J. Virol., 2006-01-01;80(1):372-82. 2006-01-01 [PMID: 16352562] (ELISA Development, Porcine)
• Carter AJ, Aggarwal M, Kopia GA, Tio F, Tsao PS, Kolata R, Yeung AC, Llanos G, Dooley J, Falotico R Long-term effects of polymer-based, slow-release, sirolimus-eluting stents in a porcine coronary model. Cardiovasc. Res., 2004-09-01;63(4):617-24. 2004-09-01 [PMID: 15306217] (Western Blot, Porcine)
• Brix-Christensen V, Andersen SK, Andersen R, Mengel A, Dyhr T, Andersen NT, Schmitz O, Orskov H, Tonnesen E Acute hyperinsulinemia restrains endotoxin-induced systemic inflammatory response: an experimental study in a porcine model. Anesthesiology, 2004-04-01;100(4):861-70. 2004-04-01 [PMID: 15087621] (ELISA Development, Porcine)

FAQs for IL-6 Antibody (AF686). (Showing 1 - 1 of 1 FAQs).

1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
   • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

Bioinformatics

• Gene Symbol: IL6
• Uniprot:
  • P26893()