IL-6 Antibody [Unconjugated]

Western blot shows lysates of NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1 µg/mL of Goat Anti-Rat IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL‑6 was detected in immersion fixed NR8383 rat alveolar macrophage cell line using Goat Anti-Rat IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cyoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Recombinant Rat IL‑6 (Catalog # 506-RL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant RatIL‑6 (0.6 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506). The ND50 is typically 0.1-0.5 µg/mL.

Diverse localization of measured cytokines in P7 brains. Representative pictures (100× magnification) of immunohistochemical stainings of IL-1 beta (Α), IL-10 (B), IL-6 (C) and TNF-alpha (D). Cytokines are stained with DAB (brown color) and counterstained with cresyl violet. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23351591), licensed under a CC-BY license. Not internally tested by R&D Systems.

Diverse localization of measured cytokines in P7 brains. Representative pictures (100× magnification) of immunohistochemical stainings of IL-1 beta (Α), IL-10 (B), IL-6 (C) and TNF-alpha (D). Cytokines are stained with DAB (brown color) and counterstained with cresyl violet. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23351591), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Rt
• Applications: WB, B/N, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-6 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant rat IL-6
  Phe25-Thr211
  Accession # P20607
• Specificity: Detects rat and mouse IL-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant cotton rat IL‑6 is observed, and less than 1% cross-reactivity with recombinant human IL-6 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL6
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization <0.5 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-6 Antibody [Unconjugated]

• B-cell differentiation factor
• B-cell stimulatory factor 2
• BSF2
• BSF-2
• CDF
• CTL differentiation factor
• HSF
• hybridoma growth factor
• IFNB2
• IFN-beta-2
• IL6
• IL-6
• Interferon beta-2
• interleukin 6 (interferon, beta 2)
• interleukin BSF-2
• interleukin-6
• MGI-2A

Background

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature rat IL-6 is 187 amino acids (aa) in length that is typically expressed as a 22-28 kDA molecular weight protein. Rat IL-6 shares 39% and 85% aa sequence identity with human and mouse IL-6, respectively. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-6 Antibody (AF506)(54)

Publications using AF506

• RM Samsonraj, SF Law, A Chandra, RJ Pignolo An unbiased proteomics approach to identify the senescence-associated secretory phenotype of human bone marrow-derived mesenchymal stem cells Bone Reports, 2023-03-21;18(0):101674. 2023-03-21 [PMID: 36994454] (Human)
• Costa DVS, Shin JH, Goldbeck SM et al. Adenosine receptors differentially mediate enteric glial cell death induced by Clostridioides difficile Toxins A and B Frontiers in immunology 1/16/2023 [PMID: 36726986]
  
  Details:
  Dilutions: 1:500
• Andrea V. Loureiro, Lauro I. Moura-Neto, Conceição S. Martins, Pedro I. M. Silva, Matheus B.S. Lopes, Renata F. C. Leitão et al. Role of Pannexin-1-P2X7R signaling on cell death and pro-inflammatory mediator expression induced by Clostridioides difficile toxins in enteric glia Frontiers in Immunology 8/22/2022 [PMID: 36072579]
• Simina-Ramona Selejan, Dominik Linz, Muriel Mauz, Mathias Hohl, Anh Khoa Dennis Huynh, Thimoteus Speer et al. Renal denervation reduces atrial remodeling in hypertensive rats with metabolic syndrome Basic Research in Cardiology 2022-12-01 [PMID: 35834066] (Western Blot, Rat)
• Simina-Ramona Selejan, Dominik Linz, Muriel Mauz, Mathias Hohl, Anh Khoa Dennis Huynh, Thimoteus Speer et al. Renal denervation reduces atrial remodeling in hypertensive rats with metabolic syndrome Basic Research in Cardiology 12/1/2022 [PMID: 35834066]
• M Nasi, A De Gaetano, G Carnevale, L Bertoni, V Selleri, G Zanini, A Pisciotta, S Caramaschi, L Reggiani B, A Farinetti, A Cossarizza, M Pinti, A Manenti, AV Mattioli Effects of Energy Drink Acute Assumption in Gastrointestinal Tract of Rats Nutrients, 2022-05-04;14(9):. 2022-05-04 [PMID: 35565895] (IHC, Rat)
• K Miyazaki, S Miyazaki, T Yurube, Y Takeoka, Y Kanda, Z Zhang, Y Kakiuchi, R Tsujimoto, H Ohnishi, T Matsuo, M Ryu, R Kuroda, K Kakutani Protective Effects of Growth Differentiation Factor-6 on the Intervertebral Disc: An In Vitro and In Vivo Study Cells, 2022-03-31;11(7):. 2022-03-31 [PMID: 35406739] (In Vivo, Mouse)
• M Jiang, H Xie, C Zhang, T Wang, H Tian, L Lu, JY Xu, GT Xu, L Liu, J Zhang Enhancing fractalkine/CX3CR1 signalling pathway can reduce neuroinflammation by attenuating microglia activation in experimental diabetic retinopathy Journal of Cellular and Molecular Medicine, 2022-01-11;0(0):. 2022-01-11 [PMID: 35023309] (Western Blot, Rat)
• ZH Wang, Y Xia, Z Wu, SS Kang, JC Zhang, P Liu, X Liu, W Song, V Huin, CM Dhaenens, SP Yu, XC Wang, K Ye Neuronal ApoE4 stimulates C/EBPbeta activation, promoting Alzheimer&#039;s disease pathology in a mouse model Progress in neurobiology, 2021-12-24;209(0):102212. 2021-12-24 [PMID: 34958873] (IHC, Rat)
• Yun Feng, Xuebin Li, Jie Wang, Xiaohua Huang, Lanqing Meng, Jianmin Huang Pyruvate kinase M2 (PKM2) improve symptoms of post‐ischemic stroke depression by activating VEGF to mediate the MAPK/ERK pathway Brain and Behavior 1/1/2022 [PMID: 34898024]
• Wareham LK, Echevarria FD, Sousa JL et al. Interleukin-6 promotes microtubule stability in axons via Stat3 protein-protein interactions iScience 10/22/2021 [PMID: 34646984]
• Wareham LK, Echevarria FD, Sousa JL et al. Interleukin-6 promotes microtubule stability in axons via Stat3 protein-protein interactions iScience 2021-10-22 [PMID: 34646984]
• F Yergöz, J Friebel, N Kränkel, U Rauch-Kroe, HP Schultheis, U Landmesser, A Dörner Adenine Nucleotide Translocase 1 Expression Modulates the Immune Response in Ischemic Hearts Cells, 2021-08-19;10(8):. 8/19/2021 [PMID: 34440901]
• Jinrui Dong, Sivakumar Viswanathan, Eleonora Adami, Sebastian Schafer, Fathima F Kuthubudeen, Anissa A Widjaja et al. The pro-regenerative effects of hyperIL6 in drug-induced liver injury are unexpectedly due to competitive inhibition of IL11 signaling eLife 8/26/2021 [PMID: 34435951]
• Jinrui Dong, Sivakumar Viswanathan, Eleonora Adami, Sebastian Schafer, Fathima F Kuthubudeen, Anissa A Widjaja et al. The pro-regenerative effects of hyperIL6 in drug-induced liver injury are unexpectedly due to competitive inhibition of IL11 signaling eLife 2021-08-26 [PMID: 34435951] (Western Blot, Mouse)
• Marc Jonathan George, Nur Hayati Jasmin, Valerie Taylor Cummings, Angela Richard-Loendt, Francesca Launchbury, Kevin Woollard et al. Selective Interleukin-6 Trans-Signaling Blockade Is More Effective Than Panantagonism in Reperfused Myocardial Infarction JACC: Basic to Translational Science 5/1/2021 [PMID: 34095633]
• ZH Wang, Y Xia, P Liu, X Liu, L Edgington-, K Lei, SP Yu, XC Wang, K Ye ApoE4 activates C/EBP&amp;beta/&amp;delta-secretase with 27-hydroxycholesterol, driving the pathogenesis of Alzheimer&#039;s disease Progress in neurobiology, 2021-03-11;0(0):102032. 2021-03-11 [PMID: 33716161] (ICC, Neutralization, Western Blot, Rat)
• J Dong, S Viswanatha, E Adami, BK Singh, SP Chothani, B Ng, WW Lim, J Zhou, M Tripathi, NSJ Ko, SG Shekeran, J Tan, SY Lim, M Wang, PM Lio, PM Yen, S Schafer, SA Cook, AA Widjaja Hepatocyte-specific IL11 cis-signaling drives lipotoxicity and underlies the transition from NAFLD to NASH Nature Communications, 2021-01-04;12(1):66. 1/4/2021 [PMID: 33397952]
• Yujun Luo, Beibei Wang, Jianhua Liu, Faxin Ma, Dongling Luo, Zhongwen Zheng et al. Ginsenoside RG1 enhances the paracrine effects of bone marrow-derived mesenchymal stem cells on radiation induced intestinal injury Aging (Albany NY) 1/15/2021 [PMID: 33293477]
• Jinjie Liu, Zanhua Liu, Guoliang Liu, Kai Gao, Hengjie Zhou, Yongbo Zhao et al. Spinal cord injury and its underlying mechanism in rats with temporal lobe epilepsy Experimental and Therapeutic Medicine 1/15/2020 [PMID: 32104272]
• S Zilberman-, R Abu-Hamad, R Zarura, M Sova, Y Hachmo, M Stark, S Neuman, S Slavin, S Efrati Human mesenchymal stromal cells ameliorate complement induced inflammatory cascade and improve renal functions in a rat model of ischemia-reperfusion induced acute kidney injury PLoS ONE, 2019-09-12;14(9):e0222354. 2019-09-12 [PMID: 31513644] (Fuorescense Microscopy, Rat)
• M Yamamoto, M Kim, H Imai, Y Itakura, G Ohtsuki Microglia-Triggered Plasticity of Intrinsic Excitability Modulates Psychomotor Behaviors in Acute Cerebellar Inflammation Cell Rep, 2019-09-10;28(11):2923-2938.e8. 2019-09-10 [PMID: 31509752] (Western Blot, Rat)
• Pedro Alvarez, Oliver Bogen, Jon D. Levine Interleukin 6 decreases nociceptor expression of the potassium channel KV1.4 in a rat model of hand–arm vibration syndrome Pain 8/1/2019 [PMID: 31335655]
• ZH Wang, J Xiang, X Liu, SP Yu, FP Manfredsso, IM Sandoval, S Wu, JZ Wang, K Ye Deficiency in BDNF/TrkB Neurotrophic Activity Stimulates δ-Secretase by Upregulating C/EBP? in Alzheimer&#039;s Disease Cell Rep, 2019-07-16;28(3):655-669.e5. 2019-07-16 [PMID: 31315045] (Neutralization, Rat)
• Benoît Ranchoux, Valérie Nadeau, Alice Bourgeois, Steeve Provencher, Éve Tremblay, Junichi Omura et al. Metabolic Syndrome Exacerbates Pulmonary Hypertension due to Left Heart Disease Circulation Research 8/2/2019 [PMID: 31154939]
• H Ding, Y Dai, Y Lei, Z Wang, D Liu, R Li, L Shen, N Gu, M Zheng, X Zhu, G Zhao, Y Hu Upregulation of CD81 in trophoblasts induces an imbalance of Treg/Th17 cells by promoting IL-6 expression in preeclampsia Cell. Mol. Immunol., 2018-11-28;0(0):. 2018-11-28 [PMID: 30487550] (Neutralization, Rat)
• HC Lai, CH Lu, CS Wong, BF Lin, SM Chan, CY Kuo, ZF Wu Baicalein attenuates neuropathic pain and improves sciatic nerve function recovery in rats with partial sciatic nerve transection J Chin Med Assoc, 2018-08-20;0(0):. 2018-08-20 [PMID: 30139671] (Rat)
• Andrei V. Chernov, Albert G. Remacle, Swathi K. Hullugundi, Piotr Cieplak, Mila Angert, Jennifer Dolkas et al. Amino acid sequence conservation of the algesic fragment of myelin basic protein is required for its interaction with CDK5 and function in pain The FEBS Journal 9/1/2018 [PMID: 30079618]
• SA Chong, S Balosso, C Vandenplas, G Szczesny, E Hanon, K Claes, X Van Damme, B Danis, J Van Eyll, C Wolff, A Vezzani, RM Kaminski, I Niespodzia Intrinsic Inflammation Is a Potential Anti-Epileptogenic Target in the Organotypic Hippocampal Slice Model Neurotherapeutics, 2018-04-01;0(0):. 2018-04-01 [PMID: 29464573] (Neutralization, Rat)
• SX Chen, SK Wang, PW Yao, GJ Liao, XD Na, YY Li, WA Zeng, XG Liu, Y Zang Early CALP2 expression and microglial activation are potential inducers of spinal IL-6 upregulation and bilateral pain following motor nerve injury J. Neurochem., 2018-04-01;0(0):. 2018-04-01 [PMID: 29423951] (IHC-Fr, Rat)
• MA Godoy, LM Saraiva, LRP Carvalho, A Vasconcelo, HJV Beiral, AB Ramos, LRP Silva, RB Leal, VHS Monteiro, CV Braga, CA Araujo-Sil, LC Sinis, VB Santos, THK Brunswick, CL Alcantara, APCA Lima, NLD Cunha E Si, A Galina, A Vieyra, FG De Felice, R Mendez-Ote, ST Ferreira Mesenchymal stem cells and cell-derived extracellular vesicles protect hippocampal neurons from oxidative stress and synapse damage induced by amyloid-? oligomers J. Biol. Chem., 2017-12-28;0(0):. 2017-12-28 [PMID: 29284679] (Neutralization, Rat)
• Glyn Chidlow, John P. M. Wood, Robert J. Casson Investigations into Hypoxia and Oxidative Stress at the Optic Nerve Head in a Rat Model of Glaucoma Frontiers in Neuroscience 8/24/2017 [PMID: 28883787]
• Glyn Chidlow, John P. M. Wood, Robert J. Casson Investigations into Hypoxia and Oxidative Stress at the Optic Nerve Head in a Rat Model of Glaucoma Frontiers in Neuroscience 2017-08-24 [PMID: 28883787] (Immunohistochemistry, Rat)
• P Shrivastav, MA Cabrera, LG Chastain, NI Boyadjieva, S Jabbar, T Franklin, DK Sarkar Mu-opioid receptor and delta-opioid receptor differentially regulate microglial inflammatory response to control proopiomelanocortin neuronal apoptosis in the hypothalamus: effects of neonatal alcohol J Neuroinflammation, 2017-04-14;14(1):83. 2017-04-14 [PMID: 28407740] (Neutralization, Rat)
• T Nozu, S Miyagishi, R Nozu, K Takakusaki, T Okumura Repeated water avoidance stress induces visceral hypersensitivity; role of IL-1, IL-6 and peripheral corticotropin-releasing factor J. Gastroenterol. Hepatol, 2017-12-01;0(0):. 2017-12-01 [PMID: 28299830] (In Vivo, Rat)
• Justin S. Ko, Kelly A. Eddinger, Mila Angert, Andrei V. Chernov, Jennifer Dolkas, Alex Y. Strongin et al. Spinal activity of interleukin 6 mediates myelin basic protein-induced allodynia Brain, Behavior, and Immunity 8/1/2016 [PMID: 26970355]
• Justin S. Ko, Kelly A. Eddinger, Mila Angert, Andrei V. Chernov, Jennifer Dolkas, Alex Y. Strongin et al. Spinal activity of interleukin 6 mediates myelin basic protein-induced allodynia Brain, Behavior, and Immunity 2016-08-01 [PMID: 26970355] (In vivo assay, Rat)
• Evi Vlassaks, Tomasz Brudek, Bente Pakkenberg, Antonio W. D. Gavilanes Cerebellar Cytokine Expression in a Rat Model for Fetal Asphyctic Preconditioning and Perinatal Asphyxia The Cerebellum 8/1/2014 [PMID: 24771476]
• Daniela Farkas, Aysar A. Alhussaini, Donatas Kraskauskas, Vita Kraskauskiene, Carlyne D. Cool, Mark R. Nicolls et al. Nuclear Factor kappa B Inhibition Reduces Lung Vascular Lumen Obliteration in Severe Pulmonary Hypertension in Rats American Journal of Respiratory Cell and Molecular Biology 9/1/2014 [PMID: 24684441]
• Bustamante M, Fernandez-Verdejo R, Jaimovich E, Buvinic S Electrical stimulation induces IL-6 in skeletal muscle through extracellular ATP by activating Ca(2+) signals and an IL-6 autocrine loop. Am J Physiol Endocrinol Metab, 2014-02-11;306(8):E869-82. 2014-02-11 [PMID: 24518675] (Neutralization, Rat)
• Sauvant J, Delpech J, Palin K, De Mota N, Dudit J, Aubert A, Orcel H, Roux P, Laye S, Moos F, Llorens-Cortes C, Nadjar A Mechanisms involved in dual vasopressin/apelin neuron dysfunction during aging. PLoS ONE, 2014-02-05;9(2):e87421. 2014-02-05 [PMID: 24505289] (Neutralization, Rat)
• Shigemoto-Mogami Y, Hoshikawa K, Goldman J, Sekino Y, Sato K Microglia enhance neurogenesis and oligodendrogenesis in the early postnatal subventricular zone. J Neurosci, 2014-02-05;34(6):2231-43. 2014-02-05 [PMID: 24501362] (Neutralization, Rat)
• Zhang J, Li Y, Shan K, Wang L, Qiu W, Lu Y, Zhao D, Zhu G, He F, Wang Y Sublytic C5b-9 induces IL-6 and TGF-beta1 production by glomerular mesangial cells in rat Thy-1 nephritis through p300-mediated C/EBPbeta acetylation. FASEB J, 2013-12-16;28(3):1511-25. 2013-12-16 [PMID: 24344329] (Bioassay, Rat)
• M Leibinger, A Müller, P Gobrecht, H Diekmann, A Andreadaki, D Fischer Interleukin-6 contributes to CNS axon regeneration upon inflammatory stimulation Cell Death & Disease 4/25/2013 [PMID: 23618907]
• Kim D, Niewiesk S Synergistic induction of interferon alpha through TLR-3 and TLR-9 agonists identifies CD21 as interferon alpha receptor for the B cell response. PLoS Pathog, 2013-03-14;9(3):e1003233. 2013-03-14 [PMID: 23516365] (Neutralization, Rat)
• Furuhashi , Kazuhiro, Tsuboi , Naotake, Shimizu , Asuka, Katsuno , Takayuki, Kim , Hangsoo, Saka , Yosuke, Ozaki , Takenori, Sado , Yoshikaz, Imai , Enyu, Matsuo , Seiichi, Maruyama , Shoichi Serum-starved adipose-derived stromal cells ameliorate crescentic GN by promoting immunoregulatory macrophages. J Am Soc Nephrol, 2013-03-07;24(4):587-603. 2013-03-07 [PMID: 23471196] (Neutralization, Rat)
• Vlassaks E, Strackx E, Vles J, Nikiforou M, Martinez-Martinez P, Kramer B, Gavilanes A Fetal asphyctic preconditioning modulates the acute cytokine response thereby protecting against perinatal asphyxia in neonatal rats. J Neuroinflammation, 2013-01-26;10(0):14. 2013-01-26 [PMID: 23351591] (IHC-Fr, Rat)
• Oh J, McCloskey MA, Blong CC Astrocyte-derived interleukin-6 promotes specific neuronal differentiation of neural progenitor cells from adult hippocampus. J. Neurosci. Res., 2010-10-01;88(13):2798-809. 2010-10-01 [PMID: 20568291] (Neutralization, Rat)
• Murase S, Terazawa E, Queme F, Ota H, Matsuda T, Hirate K, Kozaki Y, Katanosaka K, Taguchi T, Urai H, Mizumura K Bradykinin and nerve growth factor play pivotal roles in muscular mechanical hyperalgesia after exercise (delayed-onset muscle soreness). J. Neurosci., 2010-03-10;30(10):3752-61. 2010-03-10 [PMID: 20220009] (Neutralization, Rat)
• Dominguez E, Rivat C, Pommier B, Mauborgne A, Pohl M JAK/STAT3 pathway is activated in spinal cord microglia after peripheral nerve injury and contributes to neuropathic pain development in rat. J. Neurochem., 2008-07-12;107(1):50-60. 2008-07-12 [PMID: 18636982] (Neutralization, Rat)
• Xu J, Kim HT, Ma Y, Zhao L, Zhai L, Kokorina N, Wang P, Messina JL Trauma and hemorrhage-induced acute hepatic insulin resistance: dominant role of tumor necrosis factor-alpha. Endocrinology, 2008-01-10;149(5):2369-82. 2008-01-10 [PMID: 18187553] (Neutralization, Rat)
• Nakanishi M, Niidome T, Matsuda S, Akaike A, Kihara T, Sugimoto H Microglia-derived interleukin-6 and leukaemia inhibitory factor promote astrocytic differentiation of neural stem/progenitor cells. Eur. J. Neurosci., 2007-02-01;25(3):649-58. 2007-02-01 [PMID: 17328769] (Immunodepletion, Rat)
• Barkho BZ, Song H, Aimone JB Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation. Stem Cells Dev., 2006-06-01;15(3):407-21. 2006-06-01 [PMID: 16846377] (Neutralization, Rat)
• Yang S, Hu S, Hsieh YC, Choudhry MA, Rue LW, Bland KI, Chaudry IH Mechanism of IL-6-mediated cardiac dysfunction following trauma-hemorrhage. J. Mol. Cell. Cardiol., 2006-02-24;40(4):570-9. 2006-02-24 [PMID: 16499925] (Neutralization, Rat)

Review for IL-6 Antibody (AF506) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer IF Rat 01/08/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 22469908
• Species: Rat

FAQs for IL-6 Antibody (AF506). (Showing 1 - 1 of 1 FAQs).

1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
   • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

Bioinformatics

• Gene Symbol: IL6
• Uniprot:
  • P20607()