| Reactivity | Hu, RM, PmSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF |
| Clone | MQ2-13A5 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | DyLight 550 |
| Immunogen | COS-expressed human IL-6 |
| Specificity | Human/Rhesus/Cynomolgus IL-6 |
| Predicted Species | Monkey (100%). Backed by our 100% Guarantee. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | IL6 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
![IL-6 Antibody (MQ2-13A5) [DyLight 550]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "IL-6 Antibody (MQ2-13A5) [DyLight 550]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-33047AF350 | |
| Alexa Fluor 405 | NBP2-33047AF405 | |
| Alexa Fluor 488 | NBP2-33047AF488 | |
| Alexa Fluor 532 | NBP2-33047AF532 | |
| Alexa Fluor 594 | NBP2-33047AF594 | |
| Alexa Fluor 647 | NBP2-33047AF647 | |
| Alexa Fluor 700 | NBP2-33047AF700 | |
| Alexa Fluor 750 | NBP2-33047AF750 | |
| Allophycocyanin | NBP2-33047APC | |
| Biotin | NBP2-33047B | |
| DyLight 350 | NBP2-33047UV | |
| DyLight 405 | NBP2-33047V | |
| DyLight 488 | NBP2-33047G | |
| DyLight 550 | NBP2-33047R | |
| DyLight 594 | NBP2-33047DL594 | |
| DyLight 650 | NBP2-33047C | |
| DyLight 680 | NBP2-33047FR | |
| DyLight 755 | NBP2-33047IR | |
| FITC | NBP2-33047F | |
| HRP | NBP2-33047H | |
| Janelia Fluor 549 | NBP2-33047JF549 | |
| Janelia Fluor 646 | NBP2-33047JF646 | |
| PE | NBP2-33047PE | |
| PerCP | NBP2-33047PCP |
Diseases for IL-6 Antibody (NBP2-33047R)Discover more about diseases related to IL-6 Antibody (NBP2-33047R).
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PTMs for IL-6 Antibody (NBP2-33047R)Learn more about PTMs related to IL-6 Antibody (NBP2-33047R).
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Rest in Peace: Is the Receptor Interacting Protein (RIP) Kinase-3 (RIPK3) a Protector or Offender? By Jamshed Arslan, Pharm. D., PhD. Receptor interacting protein kinases (RIPKs) in necroptosisDeath is perhaps inevitable. Cell death can be programed and immunologically silent (apoptosis), unprogrammed and inflamm... Read full blog post. |
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Gut-brain axis: microbiota influence behavior and mental well-being By Jennifer Sokolowski, MD, PhD.Gut-microbiome interactionsOn and in our bodies, microbes outnumber our cells by about ten-to-one. Studies have revealed that the microbiome influences neurogenesis, cognition, and... Read full blog post. |
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Metabolic Syndrome: Symptoms and associated disease states By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven of the Stanford University, CA, explained the relationship between four diseases:... Read full blog post. |
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Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN... Read full blog post. |
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The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins. The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein... Read full blog post. |
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The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura. It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol... Read full blog post. |
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VEGF: Vascular endothelial growth factor VEGF is homodimeric, disulfide-linked glycoprotein cytokine that serves as the ligand for FLT1 (VEGFR-1 receptor) and FLK1 (VEGFR-2 receptor) tyrosine kinases. It is a key modulator of physiological angiogenesis, vasculogenesis, and endothelial cell g... Read full blog post. |
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TLR2 - I can recognize many foreign pathogens! TLR2 is a member of the broad family of Toll-like receptors (TLR) that play an important role in pathogen recognition and innate immunity. TLRs, like other pattern recognition receptors (PRRs), recognize endogenous ligands released during cell or tiss... Read full blog post. |
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MyD88 Antibodies for IL Signaling and Immunity Research The myeloid differentiation protein MyD88 (myeloid differentiation primary response protein) was originally identified and characterized as a primary upregulated response gene in interleukin-6 mediated myeloid differentiation. Now, MyD88 is known to b... Read full blog post. |
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"Get the skinny" on Adiponectin & Leptin Antibodies We at Novus Biologicals have an extensive antibody catalog covering lipid and metabolism research, including Adiponectin and leptin antibody products.Excess body fat is linked to many human diseases, including IRS (insulin resistance syndrome), type... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | IL6 |
![Western Blot: STAT3 Antibody (9D8) [NBP2-22471] - Human breast cancer cell MDA-MB-231 was treated with carboplatin for 72 hours and the expression of p-Stat3 at Y705 and total Stat3 were detected by western blot. From verified customer review.](http://images.novusbio.com/images2/STAT3-Antibody-(9D8)-Western-Blot-NBP2-22471-img0023.jpg)
![Immunocytochemistry/Immunofluorescence: STAT3 Antibody (9D8) [NBP2-22471] - Analysis of STAT3 using anti-STAT3 (9D8) monoclonal antibody (shown in green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing STAT3, at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/images2/STAT3-Antibody-(9D8)-Immunocytochemistry-Immunofluorescence-NBP2-22471-img0016.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (9D8) [NBP2-22471] - Biopsies of normal and cancer tissues.](http://images.novusbio.com/images2/STAT3-Antibody-(9D8)-Immunohistochemistry-Paraffin-NBP2-22471-img0017.jpg)
![Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - LIF expression in human primary T-cells and cancer cell lines: Jurkat and K562. Image from verified customer review.](http://images.novusbio.com/images2/LIF-Antibody-(39N7D10)-Western-Blot-NBP2-27406-img0009.jpg)
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10).](http://images.novusbio.com/images2/LIF-Antibody-(39N7D10)-Immunohistochemistry-Paraffin-NBP2-27406-img0008.jpg)
![Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - WB validation of LIF antibody (clone 39N7D10) on (A) full-length recombinant Lif protein, (B) mouse spleen lysate and (C) human spleen lysate. 3 ug/mL concentration of primary antibody, Goat anti-rat IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this assay.](http://images.novusbio.com/images2/LIF-Antibody-(39N7D10)-Western-Blot-NBP2-27406-img0001.jpg)
![Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.](http://images.novusbio.com/images2/NFkB-p105-p50-Antibody-(2J10D7)-Western-Blot-NB100-56583-img0004.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - IHC-P of rabbit aorta using NB100-56583. Secondary antibody: anti-mouse histofine ( Nisherei Bioscience Inc. ref: 414131F). Development: DAB (Dako ref: K346811-2) and counterstained with Hematoxylin. Submitted via verified customer review](http://images.novusbio.com/images2/NFkB-p105-p50-Antibody-(2J10D7)-Immunohistochemistry-Paraffin-NB100-56583-img0009.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U937 cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/images2/NFkB-p105-p50-Antibody-(2J10D7)-Flow-Cytometry-NB100-56583-img0008.jpg)
![Dual RNAscope ISH-IHC: TLR4 Antibody (76B357.1) [NB100-56566] - FFPE tissue sections of human tonsil were probed for TLR4 mRNA (ACD RNAScope Probe, ACD catalog # 311281; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Mouse Monoclonal (Novus Biologicals catalog # NB100-56566) at 5ug/mL with 1 hour incubation at room temperature followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.](http://images.novusbio.com/images2/TLR4-Antibody-(76B357.1)-Dual-RNAscope-ISH-IHC-NB100-56566-img0036.jpg)
![Flow Cytometry: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-27149. TLR4 expression on monocytes from human peripheral blood. PBMC were stained in a 2 color flow test, with CD14 PE version of this antibody and 1 ug of either isotype control (left) or TLR4-Alexa Fluor 647 (right). PPI negative, CD14+ cells were gated for analysis.](http://images.novusbio.com/images2/TLR4-Antibody-(76B357.1)-Flow-Cytometry-NB100-56566-img0024.jpg)
![Immunohistochemistry: TLR4 Antibody (76B357.1) [NB100-56566] - Pericryptal Myofibroblasts are Responsible for Increased TLR4 Expression in a Subset of CRCs. Double-stained immunofluorescence for TLR4 (green) and vimentin (red) in normal (I), adenoma (II), and colon adenocarcinoma (III) (10x). In the stromal compartment of CRCs, immunofluorescent staining for TLR4 localized to the pericryptal myofibroblasts in a subset of samples. Image collected and cropped by CiteAb from the following publication (http://www.jeccr.com/content/33/1/45), licensed under a CC-BY licence.](http://images.novusbio.com/images2/TLR4-Antibody-(76B357.1)-Immunohistochemistry-NB100-56566-img0040.jpg)
![IL-6 [HRP]](http://images.novusbio.com/images2/IL6_D6050_ELISA_555.jpg "IL-6 [HRP]")