IL-6 Antibody (IL6/1270)

Immunohistochemistry-Paraffin: IL-6 Antibody (IL6/1270) [NBP2-44953] - Human Colon Carcinoma stained with IL-6 Monoclonal Antibody (IL6/1270).

Immunohistochemistry-Paraffin: IL-6 Antibody (IL6/1270) [NBP2-44953] - Human Esophageal Carcinoma stained with IL-6 Monoclonal Antibody (IL6/1270).

• Reactivity: Hu, Mu
• Applications: Flow, ICC/IF, IHC, IHC-P, ICC/IF
• Clone: IL6/1270
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

IL-6 Antibody (IL6/1270) Summary

• Immunogen: Recombinant IL-6 protein
• Localization: Cytoplasmic and extracellular (secreted)
• Specificity: IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B-cells and T-cells. It plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. It is involved in host defense, acute phase reactions, immune responses, and hematopoiesis.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: IL6
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
• Application Notes: Hu-chromosome location: 7p21
  Immunohistochemistry-Paraffin 1 - 2 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10 - 20 min followed by cooling at RT for 20 minutes. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30799288).
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10mM PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for IL-6 Antibody (IL6/1270)

• B cell stimulatory factor-2
• B-cell differentiation factor
• BSF2
• BSF-2
• BSF2CTL differentiation factor
• CDF
• HGFHSFIFNB2Hybridoma growth factor
• IFNB2
• IFN-beta-2
• IL6
• IL-6
• IL-6B-cell stimulatory factor 2
• Interferon beta-2
• interleukin 6 (interferon, beta 2)
• interleukin BSF-2
• interleukin-6
• MGI-2A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-6 Antibody (NBP2-44953)(2)

Publications using NBP2-44953

• Thompson PJ, Shah A, Apostolopolou CC, Bhushan A BET proteins are required for transcriptional activation of the senescent beta cell secretome in Type 1 Diabetes bioRxiv Aug 15 2019 (IHC-P, Mouse, Human)
• Thompson PJ, Shah A, Ntranos V et al. Targeted Elimination of Senescent Beta Cells Prevents Type 1 Diabetes Cell Metab. Feb 14 2019 [PMID: 30799288] (ICC/IF, Mouse)

FAQs for IL-6 Antibody (NBP2-44953). (Showing 1 - 1 of 1 FAQs).

1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
   • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

Bioinformatics

• Gene Symbol: IL6
• Uniprot:
  • P05231()