IL-5 Antibody [Alexa Fluor™ Plus 555]

• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor Plus 555

IL-5 Antibody [Alexa Fluor™ Plus 555] Summary

• Specificity: Detects canine canine IL‑5 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Interleukin-5 (IL-5) is a 40‑45 kDa secreted disulfide-linked homodimeric glycoprotein that plays an important role in the differentiation, growth, and function of eosinophils. It also primes basophils for histamine and leukotriene release. In mice, IL-5 also induces the proliferation, differentiation, and immunoglobulin production of B cells especially B-1 cells that constitutively express IL-5 receptor alpha . IL-5 is primarily produced by CD4⁺ Th2 cells. Other cell types, including mast cells, visceral smooth muscle cells, bronchial epithelium, CD16⁺ NK cells, eosinophils and gamma δ T cells, can also produce IL-5. Canine IL-5 is synthesized as a 134 amino acid (aa) precursor that contains a 21 aa signal sequence and a 113 aa mature segment. Mature canine IL-5 shares 62%, 66%, 85%, 84%, 58%, and 56% aa sequence identity with mature human, guinea pig, porcine, feline, mouse, and rat IL-5, respectively. The receptor for IL-5 consists of a 60 kDa ligand-binding subunit (IL-5 R alpha ) and a 120 kDa signal-transducing subunit ( beta _c) (1‑7).

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3. Takatsu, K. (1998) Cytokine Growth Factor Rev. 9:25.
4. Zabeau, L. et al. (2003) Curr. Drug Targets Inflamm. Allergy 2:319.
5. Yang, S. et al. (2001) J. Interferon Cytokine Res. 21:361.
6. Jans, D.A. and G. Hassan (1998) BioEssays 20:400.
7. Geijsen, N. et al. (2001) Cytokine Growth Factor Rev. 12:19.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.