IL-5 Antibody [Alexa Fluor® 488]

• Reactivity: Fe
• Applications: WB, B/N, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

IL-5 Antibody [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant feline IL-5
  Ile20-Ser134
  Accession # O77515
• Specificity: Detects feline IL-5 in direct ELISAs and Western blots. In direct ELISAs, approximately 80% cross-reactivity with recombinant bovine IL-5 and recombinant equine IL-5 is observed, approximately 50% cross-reactivity with recomb
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Neutralization
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-5 Antibody [Alexa Fluor® 488]

• BCDF mu
• B-cell differentiation factor I
• BCGFII
• EDF
• Eo-CSF
• Eosinophil differentiation factor
• IL5
• IL-5
• IL-5T-cell replacing factor
• interleukin 5 (colony-stimulating factor, eosinophil)
• interleukin-5
• TRF
• TRFB cell differentiation factor I

Background

Interleukin 5 (IL-5) is a T cell-derived factor that promotes the proliferation, differentiation and activation of eosinophils. In mice, IL-5 is also a growth and differentiation factor for B cells (1‑3). Various names previously used to describe IL-5 include: T cell replacing factor (TRF), B cell growth factor II (BCGFII), B cell differentiation factor μ (BCDF μ), eosinophil differentiation factor (EDF) and eosinophil colony-stimulating factor (E_o-CSF). Biologically active IL-5 is a disulfide-linked homodimer. As in human IL-5, the cDNA for cat IL-5 encodes a precursor protein with signal peptide that is cleaved to generate the secreted mature protein containing 115 amino acid (aa) residues. Feline IL-5 shares 70% and 59% aa sequence identity with human and mouse IL-5, respectively. IL-5 exerts its activity on target cells by binding to specific cell surface receptor complexes. The functional high-affinity receptor complex for IL-5 is composed of a ligand-binding alpha subunit that is specific for IL-5, and a non ligand-binding common beta subunit that is required for signal transduction. The common beta subunit is shared with the high-affinity receptor complexes for IL-3 and GM-CSF. In human, IL-5 R alpha subunit is primarily expressed on eosinophils and basophils. During eosinophil development, IL-5 up‑regulates the expression of IL-5 R alpha . In contrast, in mature eosinophils, the expression of IL-5 R alpha mRNA is down-regulated by IL-5, as well as by IL-3 and GM-CSF. Furthermore, IL-5 also down-modulates cell surface IL-5 R alpha via a proteinase-mediated process that releases the soluble IL-5 R alpha extracellular domain (4‑6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.