IL-5 Antibody (TRFK5R) [Unconjugated]

Recombinant Mouse IL-5 (Catalog # 405-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Recombinant Human/Mouse IL-5 Monoclonal Antibody (Catalog # MAB405R). The ND50 is typically 4-15 ng/mL.

• Reactivity: Hu, Mu
• Applications: B/N, ELISA(Cap)
• Clone: TRFK5R
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

IL-5 Antibody (TRFK5R) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse T cell clone-derived partially purified recombinant mouse IL-5
• Specificity: Detects human and mouse IL-5 in ELISAs. In sandwich immunoassays with BAM6051 as the detection antibody, no cross-reactivity with recombinant human (rh) IL-3, rhGM-CSF, or recombinant mouse (rm) IL-5 is observed. In sandwich ELISAs with BAM705 as the detection antibody, no cross-reactivity with rhIL-5, rmIL-3, or rmGM-CSF is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: IL5
• Purity Statement: Protein A or G purified from cell culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • ELISA Standard (Matched Pair)
  • Neutralization 4-15 ng/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-5 Antibody (TRFK5R) [Unconjugated]

• BCDF mu
• B-cell differentiation factor I
• BCGFII
• EDF
• Eo-CSF
• Eosinophil differentiation factor
• IL5
• IL-5
• IL-5T-cell replacing factor
• interleukin 5 (colony-stimulating factor, eosinophil)
• interleukin-5
• TRF
• TRFB cell differentiation factor I

Background

IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5 specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-5 Antibody (MAB405R)(1)

Publication using MAB405R

• Kankaanranta H, Ilmarinen P, Zhang X, Adcock I, Lahti A, Barnes P, Giembycz M, Lindsay M, Moilanen E Tumour necrosis factor-alpha regulates human eosinophil apoptosis via ligation of TNF-receptor 1 and balance between NF-kappaB and AP-1. PLoS ONE, 2014-02-28;9(2):e90298. 2014-02-28 [PMID: 24587316] (Neutralization, Human)

Bioinformatics

• Gene Symbol: IL5