IL-5 Antibody (TRFK5) [Unconjugated]


Human peripheral blood monocytes treated with 50 ng/mL PMA and 500 ng/mL Calcium Ionomycin for 6 hours were stained with Rat Anti-Human/Mouse IL‑5 Monoclonal Antibody (Catalog # MAB405) followed by more
Recombinant Mouse IL‑5 (Catalog # 405-ML) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑5 (0.5 ng/mL) more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications B/N, CyTOF-reported, ELISA(Cap), Flow

Order Details

IL-5 Antibody (TRFK5) [Unconjugated] Summary

Mouse T cell clone-derived partially purified recombinant mouse IL-5
Detects human and mouse IL-5 in ELISAs. In sandwich immunoassays with BAM6051 as the detection antibody, no cross-reactivity with recombinant human (rh) IL-3, rhGM-CSF, or recombinant mouse (rm) IL-5 is observed. In sandwich ELISAs with BAM705 as the detection antibody, no cross-reactivity with rhIL-5, rmIL-3, or rmGM-CSF is observed.
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-reported
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • ELISA Standard (Matched Pair)
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.004-0.015 ug/mL
Application Notes
ELISA Detection: Mouse IL-5 Biotinylated Antibody (Catalog number BAM705)
Standard: Recombinant Mouse IL-5 (Catalog number 405-ML)
Read Publications using
MAB405 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-5 Antibody (TRFK5) [Unconjugated]

  • BCDF mu
  • B-cell differentiation factor I
  • EDF
  • Eo-CSF
  • Eosinophil differentiation factor
  • IL5
  • IL-5
  • IL-5T-cell replacing factor
  • interleukin 5 (colony-stimulating factor, eosinophil)
  • interleukin-5
  • TRF
  • TRFB cell differentiation factor I


IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5 specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: ELISA
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Species: Hu
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Species: Hu
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Applications: BA
Species: Hu
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Species: Ch
Applications: ELISA, IHC, IP, Single-Cell Western, WB
Species: Mu
Applications: ELISA
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Species: Hu
Applications: BA
Species: Hu
Applications: IHC, IHC-P, WB
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
Species: Hu
Applications: BA
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
Species: Hu, Mu
Applications: B/N, CyTOF-reported, ELISA(Cap), Flow

Publications for IL-5 Antibody (MAB405)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Guinea Pig.

We have publications tested in 3 applications: ELISA Development, Luminex Development, Neutralization.

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Luminex Development
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using MAB405 Applications Species
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012;58(2):169-77. 2012 [PMID: 22277799] (Luminex Development, Mouse) Luminex Development Mouse
Yamada T, Tani Y, Nakanishi H Eosinophils promote resolution of acute peritonitis by producing proresolving mediators in mice. FASEB J., 2011;25(2):561-8. 2011 [PMID: 20959515] (Neutralization, Mouse) Neutralization Mouse
Evidence that 13-14 di-hydro, 15-keto prostaglandin D(2)-induced airway eosinophilia in guinea-pigs is independent of interleukin-5. Inflamm. Res., 2009;58(2):103-8. 2009 [PMID: 19184361] (Neutralization, Guinea Pig) Neutralization Guinea Pig
Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines. Clin. Chem., 2007;53(11):2010-2. 2007 [PMID: 18030696] (ELISA Development, Human) ELISA Development Human
Pfleger C, Schloot N, ter Veld F Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. J. Immunol. Methods, 2007;329(1):214-8. 2007 [PMID: 17964594] (Luminex Development, Human) Luminex Development Human
Xavier-Elsas P, Santos-Maximiano E, Queto T, Mendonca-Sales S, Joseph D, Gaspar-Elsas MI, Vargaftig BB Ectopic lung transplantation induces the accumulation of eosinophil progenitors in the recipients&#039; lungs through an allergen- and interleukin-5-dependent mechanism. Clin. Exp. Allergy, 2007;37(1):29-38. 2007 [PMID: 17210039] (ELISA Development, Mouse) ELISA Development Mouse
Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin. Immunol., 2006;120(2):220-31. 2006 [PMID: 16782408] (ELISA Development, Human) ELISA Development Human
Wen H, Hogaboam CM, Gauldie J, Kunkel SL Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile. Am. J. Pathol., 2006;168(6):1940-50. 2006 [PMID: 16723709] (ELISA Development, Mouse) ELISA Development Mouse
Nygaard UC, Ormstad H, Aase A, Lovik M The IgE adjuvant effect of particles: characterisation of the primary cellular response in the draining lymph node. Toxicology, 2005;206(2):181-93. 2005 [PMID: 15588912] (ELISA Development, Mouse) ELISA Development Mouse
Couillin I, Maillet I, Jacobs M, Paesen GC, Moser R, Weston-Davies W Arthropod-derived histamine-binding protein prevents murine allergic asthma. J. Immunol., 2004;173(5):3281-6. 2004 [PMID: 15322190] (ELISA Development, Mouse) ELISA Development Mouse
Show All 13 Publications.

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Gene Symbol IL5