IL-5 Antibody (TRFK5) [Unconjugated] Summary
Mouse T cell clone-derived partially purified recombinant mouse IL-5
Detects human and mouse IL-5 in ELISAs. In sandwich immunoassays with BAM6051 as the detection antibody, no cross-reactivity with recombinant human (rh) IL-3, rhGM-CSF, or recombinant mouse (rm) IL-5 is observed. In sandwich ELISAs with BAM705 as the detection antibody, no cross-reactivity with rhIL-5, rmIL-3, or rmGM-CSF is observed.
Protein A or G purified from hybridoma culture supernatant
<0.10 EU per 1 μg of the antibody by the LAL method.
Test in a species/application not listed above to receive a full credit towards a future purchase.
CyTOF-reported ELISA Capture (Matched Antibody Pair) 2-8 ug/mL ELISA Capture (Matched Antibody Pair) 2-8 ug/mL ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL ELISA Standard (Matched Pair) ELISA Standard (Matched Pair) Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells Neutralization 0.004-0.015 ug/mL
ELISA Detection: Mouse IL-5 Biotinylated Antibody (Catalog number BAM705)
Standard: Recombinant Mouse IL-5 (Catalog number 405-ML)
Packaging, Storage & Formulations
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for IL-5 Antibody (TRFK5) [Unconjugated]
BCDF mu B-cell differentiation factor I BCGFII EDF Eo-CSF Eosinophil differentiation factor IL5 IL-5 IL-5T-cell replacing factor interleukin 5 (colony-stimulating factor, eosinophil) interleukin-5 TRF TRFB cell differentiation factor I Background
IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5 specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
for 1 year from date of receipt.
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Applications: ELISA, IHC, IP, Single-Cell Western, WB
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Applications: IHC, IHC-P, WB
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
Species: Hu, Mu
Applications: B/N, CyTOF-reported, ELISA(Cap), Flow
Publications for IL-5 Antibody (MAB405)(13)
We have publications tested in 3 confirmed species: Human, Mouse, Guinea Pig.
We have publications tested in 3 applications: ELISA Development, Luminex Development, Neutralization.
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Show All 13 Publications.
Publications using MAB405
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012;58(2):169-77. 2012 [PMID: 22277799] (Luminex Development, Mouse) Luminex Development
Yamada T, Tani Y, Nakanishi H Eosinophils promote resolution of acute peritonitis by producing proresolving mediators in mice. FASEB J., 2011;25(2):561-8. 2011 [PMID: 20959515] (Neutralization, Mouse) Neutralization
Evidence that 13-14 di-hydro, 15-keto prostaglandin D(2)-induced airway eosinophilia in guinea-pigs is independent of interleukin-5. Inflamm. Res., 2009;58(2):103-8. 2009 [PMID: 19184361] (Neutralization, Guinea Pig) Neutralization
Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines. Clin. Chem., 2007;53(11):2010-2. 2007 [PMID: 18030696] (ELISA Development, Human) ELISA Development
Pfleger C, Schloot N, ter Veld F Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. J. Immunol. Methods, 2007;329(1):214-8. 2007 [PMID: 17964594] (Luminex Development, Human) Luminex Development
Xavier-Elsas P, Santos-Maximiano E, Queto T, Mendonca-Sales S, Joseph D, Gaspar-Elsas MI, Vargaftig BB Ectopic lung transplantation induces the accumulation of eosinophil progenitors in the recipients' lungs through an allergen- and interleukin-5-dependent mechanism. Clin. Exp. Allergy, 2007;37(1):29-38. 2007 [PMID: 17210039] (ELISA Development, Mouse) ELISA Development
Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin. Immunol., 2006;120(2):220-31. 2006 [PMID: 16782408] (ELISA Development, Human) ELISA Development
Wen H, Hogaboam CM, Gauldie J, Kunkel SL Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile. Am. J. Pathol., 2006;168(6):1940-50. 2006 [PMID: 16723709] (ELISA Development, Mouse) ELISA Development
Nygaard UC, Ormstad H, Aase A, Lovik M The IgE adjuvant effect of particles: characterisation of the primary cellular response in the draining lymph node. Toxicology, 2005;206(2):181-93. 2005 [PMID: 15588912] (ELISA Development, Mouse) ELISA Development
Couillin I, Maillet I, Jacobs M, Paesen GC, Moser R, Weston-Davies W Arthropod-derived histamine-binding protein prevents murine allergic asthma. J. Immunol., 2004;173(5):3281-6. 2004 [PMID: 15322190] (ELISA Development, Mouse) ELISA Development
Kobayashi T, Iijima K, Kita H Marked airway eosinophilia prevents development of airway hyper-responsiveness during an allergic response in IL-5 transgenic mice. J. Immunol., 2003;170(11):5756-63. 2003 [PMID: 12759459] (Neutralization, Mouse) Neutralization
Tomaki M, Zhao LL, Sjostrand M, Linden A, Ichinose M, Lotvall J Comparison of effects of anti-IL-3, IL-5 and GM-CSF treatments on eosinophilopoiesis and airway eosinophilia induced by allergen. Pulm Pharmacol Ther, 2002;15(2):161-8. 2002 [PMID: 12090790] (Neutralization, Mouse) Neutralization
Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity. Am. J. Pathol., 2002;160(4):1353-60. 2002 [PMID: 11943720] (ELISA Development, Mouse) ELISA Development
Show All 13 Publications.
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